Laboratory Information
| Name | EV View on WormBase |
|---|---|
| Allele designation | ef |
| Head | Christian Reinhold Eckmann |
| Institution | Martin Luther University, Halle-Wittenberg, Halle, Germany |
| Address | Eckmann Labor, Entwicklungsgenetik (Biozentrum) Institut für Biologie/ Bereich Genetik Weinbergweg 22 Halle (Saale) 06120 Germany |
| Website | http://www.biologie.uni-halle.de/institutsbereich_genetik/heisenberg-fellow/2954640_3004625/ |
| Gene classes | dhfr gls panl skih angl parn |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| EV190 | gld-4(ef15) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Homozygous nearly sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ef15 homozygotes (pale, nearly sterile, lays few eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Schmid M, et al. Genes Dev. 2009 Apr 1;23(7):824-36. |
| EV232 | gck-3(tm1296)/dpy-21(e428) V. | C. elegans | Heterozygotes are wild-type and segregate wild-type (heterozygotes), Dpy, and sterile tm1296 homozygotes. Dev Biol. 2010 Aug 15;344(2):758-71. |
| EV247 | nhr-114(ef24) V. | C. elegans | Reference: Gracida X, Eckmann CR. Curr Biol. 2013 Apr 8;23(7):607-13. |
| EV343 | unc-119(ed3); efEx12. | C. elegans | efEx12 [glp-1::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Pick non-Unc to maintain. GFP expression in the proliferative region of the germ line (resembling endogenous GLP-1 protein localization), and also in spermatheca and other somatic tissues. Derived by bombarding strain DP38 with LAP-tagged glp-1 fosmid (WRM0630DF02). |
| EV484 | efIs155 II. | C. elegans | efIs155 [mex-5p::rpl-4::FLAG::tbb-2 3′UTR + Cbr-unc-119(+)] II. Tagged RPL-4 can be used for ribosome purifications from germ cells. Reference: Nousch M, et al. G3 (Bethesda). 2020 Sep 3:g3.401644.2020. doi: 10.1534/g3.120.401644 |
| EV57 | gls-1(ef8) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Homozygous nearly sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ef8 homozygotes (nearly sterile, lays few eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Rybarska A, et al. PLoS Genet. 2009 May;5(5):e1000494. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ef15 | Allele | deletion | |
| ef24 | Allele | ||
| ef8 | Allele | insertion |