Laboratory Information

NameEV View on WormBase
Allele designationef
HeadChristian Reinhold Eckmann
InstitutionMartin Luther University, Halle-Wittenberg, Halle, Germany
Address Eckmann Labor, Entwicklungsgenetik (Biozentrum)
Institut für Biologie/ Bereich Genetik
Weinbergweg 22
Halle (Saale) 06120
Germany
Website http://www.biologie.uni-halle.de/institutsbereich_genetik/heisenberg-fellow/2954640_3004625/
Gene classes dhfr  gls  panl  skih  angl  parn 

Strains contributed by this laboratory

Strain Genotype Species Description
EV190 gld-4(ef15) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous nearly sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ef15 homozygotes (pale, nearly sterile, lays few eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Schmid M, et al. Genes Dev. 2009 Apr 1;23(7):824-36.
EV232 gck-3(tm1296)/dpy-21(e428) V. C. elegans Heterozygotes are wild-type and segregate wild-type (heterozygotes), Dpy, and sterile tm1296 homozygotes. Dev Biol. 2010 Aug 15;344(2):758-71.
EV247 nhr-114(ef24) V. C. elegans Reference: Gracida X, Eckmann CR. Curr Biol. 2013 Apr 8;23(7):607-13.
EV343 unc-119(ed3); efEx12. C. elegans efEx12 [glp-1::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Pick non-Unc to maintain. GFP expression in the proliferative region of the germ line (resembling endogenous GLP-1 protein localization), and also in spermatheca and other somatic tissues. Derived by bombarding strain DP38 with LAP-tagged glp-1 fosmid (WRM0630DF02).
EV346 gck-3(q733)/dpy-21(e428) V. C. elegans q733 homozygotes arrest as early larvae. Dev Biol. 2010 Aug 15;344(2):758-71.
EV484 efIs155 II. C. elegans efIs155 [mex-5p::rpl-4::FLAG::tbb-2 3′UTR + Cbr-unc-119(+)] II. Tagged RPL-4 can be used for ribosome purifications from germ cells. Reference: Nousch M, et al. G3 (Bethesda). 2020 Sep 3:g3.401644.2020. doi: 10.1534/g3.120.401644
EV57 gls-1(ef8) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous nearly sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ef8 homozygotes (nearly sterile, lays few eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Rybarska A, et al. PLoS Genet. 2009 May;5(5):e1000494.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ef15 Allele deletion
ef24 Allele
ef8 Allele insertion