Laboratory Information
Name | ET View on WormBase |
---|---|
Allele designation | ek |
Head | Edward T. Kipreos |
Institution | University of Georgia, Athens, GA |
Address | Cellular Biology / UGA 724 Biological Sciences Bldg. 120 Cedar St. Athens 30602 United States |
Website | http://cellbio.uga.edu/directory/faculty/edward-t-kipreos |
Gene classes | cand cdt cki dcaf ddb elb elc lrr skpt skr vhl zer folr |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
CB3514 | lin-23(e1883)/dpy-10(e128) II. | C. elegans | Heterozygotes are WT and segregate WT, Dpy, and Long Thin Steriles (adults lay no or few eggs). lin-23 animals have excess cell divisions in larval blast cell lineages. |
ET100 | H01G02.2(ok200) IV. | C. elegans | H01G02.2. External left primer: TGCATCCCTTTGATTCCTTC. External right primer: AAACCTGGGCGCTTTTATTT. Internal left primer: GCAATCCTTGCTTGATCCAT. Internal right primer: TGATTGCAACGTTCCATGAT. Internal WT amplicon: 3033 bp. Deletion size: 1251 bp. Deletion left flank: AAACTCACTTTTGAAACATTCGGGACCATT. Deletion right flank: GATGAAGATCATGGAACGTTGCAATCAATT. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org. |
ET113 | unc-119(ed3) III; ekIs2. | C. elegans | ekIs2 contains [pie-1p::GFP::cyb-1 + unc-119(+)]. Translational fusion of CYB-1 expressed from the pie-1 promoter and including the pie-1 3'UTR. GFP::CYB-1 expression in the proximal gonad, with staining disappearing in the zygote. Maintain at 25°C. |
ET137 | C30G12.1(ok910) II. | C. elegans | C30G12.1 Homozygous. No overt morphological or behavioral abnormalities. 1286 bp deletion. The region of cosmid C30G12 that is deleted is 39,238 - 40,523 bps (inclusive). The deletion includes an ectopic 5 bp sequence, GGTTA. The sequence crossing the deletion for ok910 is: ....GCCATGGTTAAAAGT GGTTA AAAAATTCAGTATAT... This deletion was generated by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publication resulting from its use. http://www.mutantfactory.ouhsc.edu/ |
ET182 | ekEx25. | C. elegans | ekEx25 [wrt-2p::CDC-6::GFP + rol-6(su1006)]. Pick Rollers to maintain the extrachromosomal array. |
ET263 | ddb-1(tm1769)/dpy-20(e2017) IV. | C. elegans | Heterozygotes are WT and segregate WT, Dpy and ddb-1 homozygotes, which arrest as L2-stage larvae (approx. 15%) or become sterile adults with protruding vulva (approx. 85%). |
ET507 | daf-16(mu86) I; cki-2(ok2105) II; glp-1(ar202) III. | C. elegans | Temperature-sensitive. Maintain at 15C. Animals form germline tumors that prevent fertility at restrictive temerature (25C). This is the first strain reported to be used for the isolation of germ cells for in vitro culture. This strain allows germ cells to remain viable for longer periods than other tumorous mutant strains tested. Reference: Chaudhari SH, et al. Dev Cell. 2016 Jul 11;38(1):33-46. |
ET65 | cul-2(ek1)/unc-64(e246) III. | C. elegans | Heterozygotes are WT and segregate WT, Unc and adult steriles (cul-2 homozygotes). cul-2 homozygotes have large germ cells and lay few eggs (slightly temperature sensitive, with more eggs at lower temperatures) that arrest early in development with multiple nuclei. |
NJ582 | cul-1(e1756)/unc-69(e587) III. | C. elegans | Heterozygotes are WT and segregate WT, Unc and Long Thin Steriles (that arrest before the adult stage). cul-1 animals have excess cell divisions in post-L1 blast cell lineages. cul-1 was formerly known as lin-19. |
Alleles contributed by this laboratory
Allele | Type | DNA Change | Protein Change |
---|---|---|---|
ek1 | Allele |