| DZ205 |
dsh-2(ez25)/mIn1 [mIs14 dpy-10(e128)] II; him-8(e1489) IV. |
C. elegans |
mIs14 [myo-2p::GFP + pes-10p::GFP]. Him. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and Egl non-GFP ez25 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. |
| DZ224 |
him-8(e1489) IV; ezIs1 X. |
C. elegans |
ezIs1[K09C8.2::GFP + rol-6(su1006)]. ezIs1 was integrated with pPD95.65(K09C8.2 promoter and unc-54 3') and pRF4. Worms are 100% Rollers. GFP is expressed in male seminal vesicle and vas deferens cells. No expression in the hermaphrodite gonad is observed. |
| DZ240 |
fkh-6(ez16)/mIn1 [dpy-10(e128) mIs14] II; him-8(e1489) IV. |
C. elegans |
Heterozygotes are WT and GFP+ in the pharynx. mIn1[dpy-10(e128) mIs14] homozygotes are Dpy and GFP+ in the pharynx. Homozygous fkh-6(ez16) hermaphrodites are sterile and have gonadogenesis defects. Homozygous fkh-6(ez16) males are sterile and strong Gon, "white patch" phenotype. 25% of males have a hermaphrodite vulval structure. |
| DZ325 |
ezIs2 III; him-8(e1489) IV. |
C. elegans |
ezIs2 [fkh-6::GFP + unc-119(+)]. ezIs2 was integrated with pPD95.69 (fkh-6 promoter and unc-54 3') and pMM106b (unc-119(+)). Worms are 100% non-Unc (the unc-119 background has been crossed out). GFP expression in adult hermaphrodite spermatheca is bright and weak staining is also observed in the proximal sheath cells. Weak GFP staining is also observed in Z1/Z4 cells in both sexes. |
| DZ390 |
ezIs10 II; unc-119(ed3) III; him-8(e1489) IV. |
C. elegans |
ezIs10 [(pWY3) lin-32::GFP + unc-119(+)]. |
| DZ393 |
mab-3(e1240) unc-4(e120) ref-1(ez11) II; him-8(e1489) IV. |
C. elegans |
Unc (cannot back). WT male tail. |
| DZ683 |
tra-2(ar221) II; xol-1(y9) X; rdIs4. |
C. elegans |
rdIs4 [ehn-3a::Venus(delta)]. Temperature-sensitive. Must be maintained at 15°C to produce progeny. Strain develops as hermaphrodites at 15°C (some animals are intersex with male tails), and develops as XX pseudomales at 25°C. GFP expressed in gonadal precursors. Reference: Kroetz MB & Zarkower D. G3 (Bethesda). 2015 Oct 23;5(12):2831-41. |
| DZ685 |
xol-1(y9) X; rdIs4. |
C. elegans |
rdIs4 [ehn-3a::Venus(delta)]. Slightly Egl. Male lethal. GFP expressed in gonadal precursors. Reference: Kroetz MB & Zarkower D. G3 (Bethesda). 2015 Oct 23;5(12):2831-41. |
| DZ710 |
fkh-6(ez73[3xflag + Cbr-unc-119(+)]) II. |
C. elegans |
fkh-6(ez73[3xflag + Cbr-unc-119(+)]) II. |
| DZ840 |
tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. |
C. elegans |
tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. |
| DZ841 |
tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III; zuIs236. |
C. elegans |
tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. zuIs236 [his-72(1 kb 5'UTR)::BIRA::GFP::his-72(1 kb 3'UTR) + unc-119(+)]. Location of zuIs236 is not known, but is not in LG III. |