Laboratory Information

NameDW View on WormBase
Allele designationdw
HeadBoulton, Simon
InstitutionICRF Clare Hall, South Mimms, UK
Address CRUK Clare Hall DNA Damage Response Laboratory London Research Institute Blanche Lane South Mimms, Herts EN6 3LD

Website http://www.london-research-institute.org.uk/research/77
Gene classes dut  fcd  helq  rtel  rip 

Strains contributed by this laboratory

Strain Genotype Species Description
DW101 atl-1(tm853) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). C. elegans Heterozygotes are Unc and GFP+ with signal in the pharynx. atl-1(tm853) homozygotes are non-Unc, viable, GFP-, and produce 100% dead embryos. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine. nT1[unc-?(n754) let-? qIs50] is also known as DnT1[qIs50]. qIs50 is apparently inserted on DnT1. qIs50 is somewhat dimmer than the similar qIs51.
DW102 brc-1(tm1145) III. C. elegans Homozygous viable. Weak Him phenotype (2-3%). Extremely sensitive to ionizing radiation and other DNA damaging agents.
DW103 brd-1(dw1) III. C. elegans Homozygous viable. Weak Him phenotype (2-3%). Extremely sensitive to ionizing radiation and other DNA damaging agents.
DW104 brc-2(tm1086) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans tm1086 is homozygous lethal. Maternally rescued. Fails to produce viable progeny due to a defect in repairing meiotic DNA double-strand breaks. Chromosomes are visibly aggregated at diakinesis. Maintain by picking GFP progeny and checking that the non-GFP progeny that are produced fail to give viable progeny. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
dw1 Allele