Caenorhabditis Genetics Center (CGC) - College of Biological Sciences

Laboratory Information

Name	DP View on WormBase
Allele designation	ed
Head	Pilgrim, Dave
Institution	University of Alberta, Edmonton, Canada
Address	University of Alberta G-507A Biological Science Building - Genetics Wing 11355 - Saskatchewan Drive Edmonton T6G 2E9 Canada
Website	http://www.biology.ualberta.ca/pilgrim.hp/lab.html
Gene classes	pdl

Strain	Genotype	Species	Description
DP132	edIs6 IV.	C. elegans	edIs6 [unc-119::GFP + rol-6(su1006)] IV. Strong Roller phenotype. Hets are not Rollers (despite the presence of the supposedly dominant su1006 mutation in the array), so heterozygous males mate well. edIs6 is the integration of an array carrying pDP#MMUGF12 and pRF4. pDP#MMUGD12 ia an unc-119::GFP fusion that encodes 101 aa of UNC-119 and was made from the Fire lab vector pPD95.77. pRF4 is the rol-6(su1006) plasmid that gives a Rol phenotype. This strain allows the nervous system to be visualized by GFP fluorescence. GFP expression starts in the early embryo and continues through adulthood in most, if not all, of the nervous system. The expression of a similar lacZ fusion (but carrying a nuclear localizing signal) is described in Genetics 141: 977-988 1995.
DP246	unc-45(st601)/sC1 [dpy-1(s2170)] III.	C. elegans	Made from LV15. st601 is a non-conditional two-fold arrest lethal.
DP485	Ctr-dpy-10(ed73) II.	C. tropicalis	Homozygotes for this mutation express a dumpy phenotype, while heterozygotes are roller and slightly shorter-than-wildtype in length. This Ctr-dpy-10 mutation will serve as a suitable syntenic marker for chromosome II mutations in C. tropicalis that does not impact the viability or fecundity of the organism. Generated in a C. tropicalis JU1373 background. Hermaphrodite. Culture at 20°C or above.

Allele	Type	DNA Change	Protein Change
ed3	Allele	substitution	nonsense
ed9	Allele	substitution	splice_site
ed4	Allele	substitution	nonsense