Laboratory Information
| Name | BAN View on WormBase |
|---|---|
| Allele designation | bon |
| Head | Daniele Bano |
| Institution | German Centre Neurodegenerative Diseases (DZNE), Bonn, Germany |
| Address | Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) Venusberg-Campus 1 Gebaude 99 Bonn 53127 Germany |
| Website | http://www.dzne.de/ |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BAN362 | micu-1(bon20) IV/nT1[qIs51] (IV; V). | C. elegans | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP bon20 homozygotes (sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. micu-1(bon20) CRISPR/Cas9-engineereed deletion removing most of the region between exons 1-3 abrogating the expression of all predicted MICU-1 isoforms. Genotyping primers (bon20 allele): Fwd GGTGGATGATCCGGGATAC, Rev CAGTGGCCATGGGCACC; micu-1(bon20): ~675 bp; WT: no product unless using longer extension time. Reference: Jackson J, et al. Mol Metab. 2022 Jul:61:101503. doi: 10.1016/j.molmet.2022.101503. PMID: 35452878. |
| BAN456 | wah-1(bon89) III. | C. elegans | wah-1(bon89) is a hypomorphic allele generated by a CRISPR/Cas9-engineered in-frame deletion. Genotyping primers: CCCACTCTCCGTTGATGGCT and TGCTTAGCCTCCTCACTGGC; wah-1(bon89)-specific product: 229 bp; WT: NO PRODUCT. Reference: Mondal M, et al. 2025 Dec 1;16(1):10817. doi: 10.1038/s41467-025-66900-8. PMID: 41326385. |
| BAN500 | micu-1(bon20) IV/nT1[qIs51] (IV;V); emre-1(bon78) X. | C. elegans | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP bon20 homozygotes (sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. micu-1(bon20) CRISPR/Cas9-engineereed deletion removing most of the region between exons 1-3 abrogating the expression of all predicted MICU-1 isoforms. Genotyping primers (bon20 allele): Fwd GGTGGATGATCCGGGATAC, Rev CAGTGGCCATGGGCACC; micu-1(bon20): ~675 bp; WT: no product unless using longer extension time. emre-1(bon78) is a CRISPR/Cas9-engineered insertion into exon 2. Genotyping primer for emre-1(bon78): caacagcccccggcggcgt and catcgtcgtcttcatcggttggcac; emre-1(bon78): 221 bp; WT: 200 bp. Reference: Jackson J, et al. Mol Metab. 2022 Jul:61:101503. doi: 10.1016/j.molmet.2022.101503. PMID: 35452878. |
| BAN544 | tag-321(bon103) IV. | C. elegans | tag-321(bon103) is a CRISPR/Cas9-engineered 1.1 kb deletion. Genotyping primers: GGTTGTTTTCCAGTCAAATCCCTCTCAC and GCACATTGAAGTTTTGAGCAAATAACAGGG; WT: ~1330 bp; bon103: ~160 bp. Reference: Vetrella M, et al. 2025 Dec 17;17(1):923. doi: 10.1038/s41467-025-67647-y. PMID: 41408045. |
| BAN545 | C03B8.3(bon104) III. | C. elegans | C03B8.3(bon104) is a CRISPR/Cas9-engineered 5 kb deletion. Genotyping primers: GATGTACGCTCACGCGAAAAATACG and AGCAAGAAGAGATCAAAACCATTTAATCACCAC. WT: ~5 kb; bon103: ~280 bp. Reference: Vetrella M, et al. 2025 Dec 17;17(1):923. doi: 10.1038/s41467-025-67647-y. PMID: 41408045. |
| BAN547 | C03B8.3(bon104) III; tag-321(bon103) IV. | C. elegans | Derived form parental strains BAN544 and BAN545. Reference: Vetrella M, et al. 2025 Dec 17;17(1):923. doi: 10.1038/s41467-025-67647-y. PMID: 41408045. |
| BAN580 | C03B8.3(bon104) III; mcu-1(ju1154) IV; tag-321(bon105) IV. | C. elegans | tag-321(bon105) is a CRISPR/Cas9-engineered 1.1 kb deletion in mcu-1(ju1154) mutant background, replicating the tag-321(bon103) lesion. The resulting double mutant was crossed with C03B8.3(bon104) males to generate the triple mutant. tag-321(bon105) genotyping primers: GGTTGTTTTCCAGTCAAATCCCTCTCAC and GCACATTGAAGTTTTGAGCAAATAACAGGG; WT: ~1330 bp; bon103: ~160 bp. Reference: Vetrella M, et al. 2025 Dec 17;17(1):923. doi: 10.1038/s41467-025-67647-y. PMID: 41408045. |
| BAN713 | bcl-11(bon125) V. | C. elegans | bcl-11(bon125) is a CRISPR/Cas9-engineered allele causing a frameshift mutation resulting in a variant with intact C2H2-type Znf domains but with an insertion of ∼64 amino acids after proline 592. Genotyping primers: Fw CGACGTGCTTCACCGGAAGA and Rev GGCGTAGTCTGGGACATCATATGGA; bcl-11(bon125): 207 bp; WT: NO PRODUCT. Reference: Niedworok P, et al. 2026 Jan 7;29(2):114422. doi: 10.1016/j.isci.2025.114422. PMID: 41641102. |
| BAN728 | bcl-11(bon144) V. | C. elegans | bcl-11(bon144) is an in-frame insertion/deletion that was generated by CRISPR/Cas9-mediated gene editing. Genotyping primers:Fw GGGGGTTTCTGGTGTCGAATCAAC and Rev CCTTCTCAATGAAGTTAGAGAAGCAAGAAAGG; bcl-11(bon144): 184 bp; WT: NO PRODUCT. Reference: Niedworok P, et al. 2026 Jan 7;29(2):114422. doi: 10.1016/j.isci.2025.114422. PMID: 41641102. |
| BAN797 | lrk-1(bon150) I. | C. elegans | lrk-1(bon150) is a CRISPR/Cas9-generated insertion causing frameshift and missense in the kinase domain. sgRNA sequence 1: TATCTACAGAGATCTCAAGT; sgRNA sequence 2: AGAAGTGTACTTCCATCAGG. Donor Homology Sequence:TCTTCATTCTGCACATATTATCTACAGAGATCTCAAGTCCGAGAACGTTCTCGGGTGGCGCTTCCCAGCCCCGTTCTCTCCGCAAACCGACGTCCTTCTTAAGCTCGGAGACTACTCTACCTCCCGTTCCGTTCTCCCGTCAGGTGGAGCAAAGGGATTCGGAGGAACGGAAGGAT. PCR primers: AGTTCAAGTTGCTCGAGCACTGG and GGAGAACGGAACGGGAGGTAGA. lrk-1(bon150)-specific product: 164 bp; WT: NO PRODUCT. Reference: Mondal M, et al. 2025 Dec 1;16(1):10817. doi: 10.1038/s41467-025-66900-8. PMID: 41326385. |
| BAN798 | lrk-1(bon151) I. | C. elegans | Homozygous viable. lrk-1(bon151) is a CRISPR/Cas9-generated missense insertion causing truncated protein variants. sgRNA sequence 1: TATCTACAGAGATCTCAAGT; sgRNA sequence 2: AGAAGTGTACTTCCATCAGG. Donor Homology Sequence:TCTTCATTCTGCACATATTATCTACAGAGATCTCAAGTCCGAGAACGTTCTCGGGTGGCGCTTCCCAGCCCCGTTCTCTCCGCAAACCGACGTCCTTCTTAAGCTCGGAGACTACTCTACCTCCCGTTCCGTTCTCCCGTCAGGTGGAGCAAAGGGATTCGGAGGAACGGAAGGAT. PCR primers: AGTTCAAGTTGCTCGAGCACTGG and AGTTCAAGTTGCTCGAGCACTGG. PCR primers: AGTTCAAGTTGCTCGAGCACTGG and GGAGAACGGAACGGGAGGTAGA. lrk-1(bon151)-specific product: 164 bp; WT: NO PRODUCT. Reference: Mondal M, et al. 2025 Dec 1;16(1):10817. doi: 10.1038/s41467-025-66900-8. PMID: 41326385. |
| BAN925 | bcl-11(bon172) V/nT1[umnIs49] (IV;V). | C. elegans | umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Heterozygotes are wild-type mKate2+ and segregate wild-type mKate2+, very few non-mKate arrested larvae (bon145 homozygotes mostly Emb), Vul mKate2+ (nT1 homozygotes) and dead eggs (aneuploids). Maintain by picking wild-type mKate2+. bcl-11(bon172) is a CRISPR/Cas9-engineered deletion causing truncation of BCL-11 lacking ∼196 amino acids and carrying a predicted triple HA sequence (3xHA) at the C terminus. Genotyping primers: Fw GTGAGCTTGCTGAGCCAGCT and Rev CGGGTAAGAGAAGATTGAGCGCAA; bcl-11(bon172): ~250 bp; WT: NO PRODUCT. Reference: Niedworok P, et al. 2026 Jan 7;29(2):114422. doi: 10.1016/j.isci.2025.114422. PMID: 41641102. |
| BAN966 | unc-119(ed3) III; bonEx174. | C. elegans | bonEx174 [drp-1p::wrmScarlet::drp-1::drp-1 3'UTR + Cbr-unc-119( + )]. Pick non-Unc to maintain. Extrachromosomal array was generated by bombardment. Reference: Mondal M, et al. 2025 Dec 1;16(1):10817. doi: 10.1038/s41467-025-66900-8. PMID: 41326385. |
This laboratory hasn't submitted any alleles to the CGC.