Laboratory Information

NameBA View on WormBase
Allele designationhc
HeadWard, Sam
InstitutionUniversity of Arizona, Tucson, AZ
Address

Website http://www.mcb.arizona.edu/wardlab/
Gene classes fer  ferl  mpl  msp  ssg  ssp  ssq  ssr  sss  sst 

Strains contributed by this laboratory

Strain Genotype Species Description
BA1013 spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. C. elegans Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line.
BA1044 spe-27(it132) spe-29(it127) IV; him-5(e1490) V. C. elegans Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating.
BA1051 spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. C. elegans Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males.
BA1061 dpy-18(e364) spe-6(hc49) ale-1(mc14)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, Sterile Dpys (Dpy is temperature sensitive), and dead eggs. ale-1 is a recessive embryonic lethal.
BA1069 F26F4.8(hc180) III. C. elegans Deletion allele of F26F4.8. Homologous to Zn finger of Drosophila ovo transcription factor. Developmental defects in hind gut, germline and vulva. HES primers 106/107 outer and HES 108/109 inner.
BA1070 cdh-5(hc181) IV. C. elegans Deletion allele of F08B4.2. Homologous to Drosophila fat cadherin. Primers HES 114/115 outer and HES 116/117 inner.
BA1073 dyf-5(hc183) I. C. elegans Deletion allele of M04C9.5 (mak-kinase). No visible phenotype in hermaphrodites. Primers HES 186/187 outer and HES 188/189 inner.
BA1083 F43G6.6(hc184) II. C. elegans Deletion allele of F43G6.6. No obvious phenotype in hermaphrodites. HES primers 162/163 outer and 164/165 inner.
BA1084 F09C12.2(hc185) II. C. elegans Deletion allele of F09C12.2. No obvious phenotype in hermaphrodites. HES primers 122/123 outer and HES 124/125 inner.
BA1090 cav-2(hc191) V. C. elegans No visible phenotype in hermaphrodites. Deletion allele of C56A3.7.
BA1091 C35D10.2(hc192) III. C. elegans No visible phenotype in hermaphrodites. Primes HES 146/146 and HES 148/149.
BA1093 snf-10(hc194) V. C. elegans Y32F6A.2 No visible phenotype in hermaphrodites. Primers: outer HES 22/228 and inner HES 229/230.
BA14 fer-14(hc14) X. C. elegans Temperature sensitive. 8% of total oocytes produced are fertilized and give rise to larvae at 16C. Almost completely sterile at 25C.
BA15 rrf-3(hc15) II. C. elegans Temperature sensitive. Maintain at 15C.
BA521 fer-1(hc1) unc-29(e1072) I. C. elegans Temperature sensitive. Maintain at 15C. Unc.
BA522 fer-1(hc13) unc-29(e1072) I. C. elegans Temperature sensitive. Maintain at 15C. Unc.
BA524 fer-1(hc1) I; him-5(e1490) V. C. elegans Temperature sensitive. Maintain at 15C. Throws males.
BA547 fer-2(hc2) III; him-5(e1490) V. C. elegans Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males.
BA562 fer-4(hc4) him-5(e1490) V. C. elegans Temperature sensitive. Maintain at 15C. Produces about 30% males.
BA585 spe-10(hc104) unc-76(e911) V. C. elegans Unc. Temperature sensitive, maintain at 16C. Average progeny: at 16C= 7 +/- 5; at 25C= 0.1 +/- .1.
BA590 spe-10(hc104) dpy-11(e224) V. C. elegans Dpy. Temperature sensitive, maintain at 16C. Average progeny at 16C= 7 +/- 5. At 25C, average progeny = 0.1 =/- .1.
BA606 spe-6(hc49) unc-25(e156) III; eDp6 (III;f). C. elegans Animals with the Duplication are WT. Animals without the Duplication are Unc and Sterile.
BA609 spe-6(hc49) vab-7(e1562) III; eDp6 (III;f). C. elegans Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile and have an abnormal tail.
BA676 spe-6(hc92) unc-32(e189) III; eDp6 (III;f). C. elegans Unc. Animals which have lost the duplications are Sterile and Unc.
BA709 fer-1(hc80) unc-29(e1072)/sDf6 I. C. elegans Heterozygotes are WT and segregate WT heterozygotes, Sterile Unc (fer-1 unc-29 homozygotes) and L1 lethals (sDf6 homozygotes). hc80 is a nonconditional sterile. hc80 sperm have short pseudopods.
BA744 spe-10(hc104) V. C. elegans Temperature sensitive, maintain at 15C. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1.
BA782 spe-10(hc104) him-5(e1490) V. C. elegans Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1.
BA784 spe-8(hc50) I. C. elegans Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile.
BA786 spe-8(hc53) I. C. elegans Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile.
BA793 spe-26(hc138) dpy-13(e184) IV. C. elegans Temperature sensitive. Fertile at 16C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage.
BA819 spe-11(hc77) I. C. elegans Temperature sensitive sterile. Paternal effect embryonic lethal. Permissive temp is 20C. Restrictive temp is 25C.
BA821 spe-26(hc138) IV. C. elegans Temperature-sensitive. Maintain at 15C. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage. Increase lifespan in males and hermaphrodites.
BA824 spe-26(hc139) dpy-20(e1282) IV. C. elegans Temperature sensitive. Partially fertile at 16C (very few progeny). Sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage.
BA825 spe-26(hc140) dpy-20(e1282)/+ IV. C. elegans Heterozygotes are WT and segregate wild-type, wild-type heterozygotes, and Sterile Dpy (spe-4 dpy-20 homozygotes). Homozygous mutants are weak Dpy and partially fertile at 15C. Sterile at 20-25C. Spermatogenesis arrests at the spermatocyte stage.
BA836 spe-26(it112) unc-22(e66) IV. C. elegans Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage.
BA837 spe-26(it112) IV. C. elegans Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage.
BA838 spe-26(hc140) IV. C. elegans Temperature sensitive. Weak Dpy and partial fertility at 15C (very few progeny). Sterile at 20C and 25C. Spermatogenesis arrests at the spermatocyte stage.
BA839 spe-26(it118) unc-22(e66) IV. C. elegans Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage.
BA840 spe-26(hc139) IV. C. elegans Temperature sensitive. Few progeny at 16C. Sterile at 20C and 25C. Spermatogenesis arrests at the spermatocyte stage.
BA843 spe-26(it118) IV. C. elegans Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage.
BA883 spe-12(hc152) I. C. elegans Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile.
BA900 spe-27(it110) IV. C. elegans Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps, males fertile. Spermatids activate "normally" in TEA. In pronase they produce spikes but never form pseudopods. Similar to spe-12 and spe-8.
BA901 spe-27(it110) dpy-20(e1282) IV. C. elegans Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA.
BA925 spe-26(hc138) dpy-20(e1282) IV. C. elegans Temperature-sensitive. Fertile and weak Dpy at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage.
BA947 spe-27(hc161) IV. C. elegans Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile.
BA959 spe-29(it127) dpy-20(e1282) IV. C. elegans Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons.
BA962 spe-29(it127) IV. C. elegans Hermaphrodites are sterile; Males are fertile. Hermaphrodites lay oocytes (produce a few fertile eggs and many oocytes). Produce viable progeny when mated to males. Hermaphrodites produce 10X more self progeny at 20C (2.5/herm) than at either 16C or 25C.
BA963 spe-27(it132) IV. C. elegans Temperature sensitive spe-27 allele. Hermaphrodites are sterile at 25C but produce 30-40 progeny/hermaphrodite at 16C. Males are fertile.
BA966 spe-27(it132) unc-22(e66) IV. C. elegans Temperature sensitive spe-27 allele. Hermaphrodites sterile at 25C; hermaphrodites produce 30-40 progeny/hermaphrodite at 16C. Males cannot mate due to the unc-22 mutation. Maintain at 15C.
BA968 spe-29(it127) unc-24(e138) IV. C. elegans Unc. Leaky sterile at 20C; tighter at 16C and 25C. Easier to propagate as if male-female strain.
BA969 spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. C. elegans Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
BA970 spe-6(hc163) III; spe-27(it132) unc-22(e66) IV. C. elegans Twitcher Unc. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
BA971 spe-27(it132) dpy-20(e1282) IV. C. elegans Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C.
BA975 spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. C. elegans spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C.
BA979 dpy-5(e61) spe-12(hc76) I; spe-6(hc163) III. C. elegans spe-6(hc163) suppresses hermaphrodite self-sterility of hc76. Dpy.
BA984 spe-6(hc163) dpy-18(e364) III. C. elegans Dpy. spe-6(hc163) suppresses self-sterility of spe-8, spe-12, spe-27, and spe-29 mutants. Causes precocious spermatide activation. Fertile between 15-25C.
BA989 spe-6(hc163) III; spe-27(it132) IV. C. elegans spe-6(hc163) suppresses the ts self-sterile phenotype of spe-27(it132). Also suppresses spe-8, spe-12, and spe-29. Causes precocious spermatid activation. Lays eggs and oocytes. Males are weakly fertile. Fertile between 15-25C.
temp_name44 spe-12(hc149) dpy-5(e61) IV. Could not get to grow. Removed 4/29/02

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
hc17 Allele
hc40 Allele substitution
hc1 Allele substitution
hc49 Allele substitution nonsense
hc76 Allele
hc180 Allele
hc181 Allele
hc183 Allele
hc184 Allele
hc185 Allele
hc191 Allele
hc192 Allele
hc194 Allele deletion
hc14 Allele
hc15 Allele
hc2 Allele
hc23 Allele
hc24 Allele substitution
hc3 Allele substitution
hc34 Allele
hc4 Allele
hc13 Allele substitution
hc104 Allele substitution nonsense
hc6 Allele
hc88 Allele substitution
hc92 Allele substitution
hc52 Allele substitution
hc80 Allele substitution
hc78 Allele substitution
hc90 Allele
hc93 Allele substitution nonsense
hc50 Allele substitution nonsense
hc53 Allele substitution
hc138 Allele
hc137 Allele
hc77 Allele
hc139 Allele
hc140 Allele
hc152 Allele
hc161 Allele substitution splice_site
hc163 Allele substitution
hc70 Allele substitution
hc61 Allele
hc57 Allele
hc58 Allele
hc59 Allele
hc60 Allele
hc62 Allele
hc65 Allele substitution
hc66 Allele
hc67 Allele
hc69 Allele
hc75 Allele substitution