Laboratory Information
| Name | AY View on WormBase |
|---|---|
| Allele designation | ac |
| Head | Alejandro Aballay |
| Institution | Oregon Health & Science University |
| Address | Oregon Health & Science University 3181 SW Sam Jackson Park Road, 6351 Richard Jones Hall Mail code: L220 Portland 97239 United States |
| Website | http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/molecular-microbiology-and-immunology/labs/aballay-lab/index.cfm |
| Gene classes | epa |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| AY1 | nol-6(ac1) II. | C. elegans | Temperature sensitive mutant. Grow at 15 to 20C. Sterile at 25C. |
| AY101 | acIs101. | C. elegans | acIs101 [F35E12.5p::GFP + rol-6(su1006)]. Rollers. Reference: (2010) J Bio Chem 285(14):10832-40. |
| AY102 | pmk-1(km25) IV; acEx102. | C. elegans | acEx102 [vha-6p::pmk-1::GFP + rol-6(su1006)]. Maintain by picking Rollers. Reference: (2010) J Bio Chem 285(14):10832-40. |
| AY131 | zcIs4 V; vit-1(ac2) X. | C. elegans | zcIs4 [hsp-4::GFP] V. vit-1(ac2) is a dominant allele that causes ER stress. Since vit-1 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY132 | zcIs4 V; vit-2(ac3) X. | C. elegans | zcIs4 [hsp-4::GFP] V. vit-2(ac3) is a dominant allele that causes ER stress. Since vit-2 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY133 | zcIs4 V; vit-4(ac4) X. | C. elegans | zcIs4 [hsp-4::GFP] V. vit-4(ac4) is a dominant allele that causes ER stress. Since vit-4 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY134 | zcIs4 V; vit-5(ac5) X. | C. elegans | zcIs4 [hsp-4::GFP] V. vit-5(ac5) is a dominant allele that causes ER stress. Since vit-5 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY135 | zcIs4 V; vit-6(ac6) X. | C. elegans | zcIs4 [hsp-4::GFP] V. vit-6(ac6) is a dominant allele that causes ER stress. Since vit-6 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY142 | vit-2(ac3) X. | C. elegans | vit-2(ac3) is a dominant allele that causes ER stress. Since vit-2 is expressed only in adult stage, ER stress is induced only in adult stage. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY143 | flp-21(ok889) V; flp-18(gk3063) X. | C. elegans | Superficially wild-type. Reference: Singh J & Aballay A. Dev Cell. 2019 Apr 8;49(1):89-99.e4. |
| AY145 | kyIs262 IV; acEx24. | C. elegans | kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. acEx24 [srbc-48p::srbc-48(cDNA) + unc-122::GFP]. Maintain by picking GFP+ to maintain array. Integrated RFP expression in AWB and AWC. Bright GFP expression in coelomocytes. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971 |
| AY146 | kyIs262 IV; acEx25. | C. elegans | kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. acEx25 [odr-1p::srbc-48::GFP]. Maintain by picking GFP+ to maintain array. Extra-chromosomal GFP expression in AWC neurons. Integrated RFP expression in AWB and AWC. acEx25 rescues srbc-48 mediated defects. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971 |
| AY147 | acEx26. | C. elegans | acEx26 [odr-1p::RFP]. Pick RFP+ to maintain. RFP expression in AWC and AWB. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971 |
| AY156 | acIs156. | C. elegans | acIs156 [vit-2p::vit-2(G839R)::GFP + myo-2p::mCherry]. In contrast to VIT-2::GFP, VIT-2(G839R)::GFP remains in the intestine and is not transported to embryos. mCherry expression in pharynx. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. |
| AY157 | gon-2(q388) I; acEx157. | C. elegans | acEx157 [gon-2p::gon-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed primarily in intestine and gonads). gon-2 expression driven by gon-2 promoter. Transgene rescues gon-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 |
| AY158 | gon-2(q388) I; acEx158. | C. elegans | acEx158 [ges-1p::gon-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed in intestinal cells). gon-2 expression driven by ges-1 promoter. Transgene rescues gon-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 |
| AY159 | gtl-2(n2618) IV; acEx159. | C. elegans | acEx159 [gtl-2p::gtl-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed primarily in the excretory cell and pharynx). gtl-2 expression driven by gtl-2 promoter. Transgene rescues gtl-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 |
| AY160 | gtl-2(n2618) IV; acEx160. | C. elegans | acEx160 [sulp-4p::gtl-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed in the excretory cell). gtl-2 expression driven by sulp-4 promoter. Transgene rescues gtl-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 |
| AY161 | mul-1(syb1027) IV. | C. elegans | F49F1.6. mul-1(syb1027) [IV:4121342..4123166] is a CRISPR/Cas9-engineered ∼1,650-bp deletion mutant of isoforms A and B (565 bp and 952 bp deleted, with generated termination codon), leaving a predicted truncated protein of 46 amino acids. Derived by out-crossing parental strain PHX1027 (Suny Biotech) with N2 six times. Reference: Hoffman CL, et al. mBio. 2020 Mar 3;11(2):e00060-20. PMID: 32127446 |
| AY162 | mul-1(syb1027) IV; acEx162. | C. elegans | acEx162 [mul-1p::mul-1::SL2::GFP + myo-2p::mCherry]. Pick mCherry+ to maintain. GFP expression in the intestine. acEx# transgene rescues mul-1(lf). mul-1(syb1027) [IV:4121342..4123166] is a CRISPR/Cas9 ∼1,650-bp deletion mutant of isoforms A and B (565 bp and 952 bp deleted, with generated termination codon), leaving a predicted truncated protein of 46 amino acids. Reference: Hoffman CL, et al. mBio. 2020 Mar 3;11(2):e00060-20. PMID: 32127446 |
| AY180 | nhr-8(ok186);Pvha-6:nhr-8 | C elegans | Pick animals with roller. The marker used was rol-6. |
| AY181 | unc-30(ok613);Punc-30::unc-30 | C elegans | Pick animals red-head animal. The marker used was myo-2:mcherry. |
| AY182 | unc-30(ok613);Prab-3::unc-30 | C elegans | Pick animals red-head animal. The marker used was myo-2:mcherry. |
| AY183 | ASG(-)_Ex[Ptax-2::CZ::ced-3(p17)::unc-54 3’UTR + Plim-6::ced-3(p15)-NZ::unc-54 3’UTR, pRF4] | C elegans | Pick animals red animal. The marker used was myo-3:mcherry. |
| AY184 | PVP(-)_Ex[Podr-2b::CZ::ced-3(p17)::unc-54 3’UTR, Punc-53::ced-3(p15)-NZ::unc-54 3’UTR, pRF4] | C elegans | Pick animals with roller. The marker used was rol-6. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ac1 | Allele | substitution |