Laboratory Information

NameAX View on WormBase
Allele designationdb
HeadMario G de Bono
InstitutionIST Austria
Address IST Austria
Am Campus 1
Maria Gugging
Klosterneuburg 3400
Austria
Website http://www2.mrc-lmb.cam.ac.uk/groups/debono/Index.html
Gene classes lnr  maco  actl 

Strains contributed by this laboratory

Strain Genotype Species Description
AX1295 gcy-35(ok769) I. C. elegans Supresses aggregation and bordering phenotypes of npr-1(null) animals.
AX1296 gcy-36(db42) X. C. elegans Supresses aggregation and bordering phenotypes of npr-1(null) animals.
AX1297 gcy-36(db66) X. C. elegans Supresses aggregation and bordering phenotypes of npr-1(null) animals.
AX1305 gcy-34(ok1012) V; npr-1(ad609) X. C. elegans Does not supress aggregation and bordering phenotypes of npr-1(null) animals.
AX1410 flp-18(db99) X. C. elegans Impaired chemotaxis and foraging behavior. Excess intestinal fat accumulation. Reduced oxygen consumption. Derived from NL4000. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385.
AX1789 dbEx719. C. elegans dbEx719 [npr-5::mCherry + unc-122p::GFP]. Pick GFP+ to maintain. mCherry expression in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB), in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Expression was also seen in head, neck, and body wall muscles. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385.
AX1791 npr-5(ok1583) V; dbEx720. C. elegans dbEx720 [npr-5::npr-5(cDNA) + unc-122p::GFP]. Pick GFP+ to maintain. Intestinal fat accumulation is similar to wild-type. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385.
AX1792 dbEx721. C. elegans dbEx721 [npr-4::mCherry + unc-122p::GFP]. Pick GFP+ to maintain. mCherry expression in the AVA and RIV neurons, possibly in BAG, in the tail neuron PQR, and in the BDU neurons. Expression was also seen in the coelomocytes, the intestine, and the rectal gland cells. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385.
AX2061 dbEx813. C. elegans dbEx813 [gcy-37p::cGi500]. Pick GFP+ animals to maintain. The cGi500 cGMP sensor is expressed in the oxygen-sensing AQR, PQR, and URX neurons. Reference: Couto A, et al. Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):E3301-10.
AX2157 tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx722. C. elegans dbEx722 [flp-17p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in BAG. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
AX2159 tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx723. C. elegans dbEx723 [gcy-32p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AQR, PQR, and URX. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
AX2161 tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx724. C. elegans dbEx724 [flp-6p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in ASE using an ASE-specific flp-6p fragment. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
AX2164 tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx725. C. elegans dbEx725 [gcy-8p::tax-2(cDNA)::SL2::GFP + gcy-32p::tax-2(cDNA)::SL2::GFP+ flp-17p::tax-2(cDNA)::SL2::GFP + flp-6p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AFD, AQR, PQR, URX, BAG, and ASE. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
AX2178 tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx726. C. elegans dbEx726 [gcy-8p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AFD. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
AX301 npr-1(ad609) lin-15B&lin-15A(n765) X; dbEx35. C. elegans dbEx35 [npr-1::GFP + lin-15(+)]. Pick Non-Muv to maintain. Solitary feeders. GFP is expressed in approximately 20 neuron types. Reference: Coates JC, de Bono M. 2002 Nature 419: 925-929.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
db42 Allele substitution
db66 Allele substitution nonsense
db99 Allele