Laboratory Information

NameALF View on WormBase
Allele designationbaf
HeadAlfred L Fisher
InstitutionUniversity of Nebraska Medical Center
Address University of Nebraska Medical Center
Fisher Lab, DRCII Room 3062
985935 Nebraska Medical Center
Omaha 68198-5935
United States
Gene classes eaf  ell  fah  mvk  tatn 

Strains contributed by this laboratory

Strain Genotype Species Description
ALF3 unc-119(ed3) III; daf-12(rh61rh411) X. C. elegans Daf-d. Unc.
ALF4 unc-119(ed3) III; daf-12(rh61rh411) X; bafIs4. C. elegans bafIs4 [daf-12 (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
ALF62 bafIs62. C. elegans bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
ALF63 unc-119(ed3) III; bafIs63. C. elegans bafIs63 [lin-42p(mut)::GFP + unc-119(+)]. lin-42p(mut)::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+); all potential DAF-12 binding sites have been mutated. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
ALF82 unc-119(ed3) III; daf-12(rh61rh411) X; bafIs62. C. elegans bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Array was crossed into strain ALF3 to create ALF82. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
ALF9 unc-119(ed3) III; daf-12(rh61rh411) X; bafIs9. C. elegans bafIs9 [daf-12::TAP (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. TAP tag inserted into daf-12 fosmid. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179.
This laboratory hasn't submitted any alleles to the CGC.