Laboratory Information

NameAG View on WormBase
Allele designationav
HeadAndy Golden
InstitutionNIDDK/NIH
Address 8 Center Dr.
Bldg. 8, Rm. 323
Bethesda 20892
United States
Website http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/GoldenAndy
Gene classes air  apc  mat  plk  sdhb  sdhd  som  wee  fitm 

Strains contributed by this laboratory

Strain Genotype Species Description
AG150 mat-2(ar104)/unc-4(e120) bli-1(e769) II. C. elegans Heterozygotes are WT and segregate WT, UncBli, and Steriles which have an everted vulva. ar104 previously called evl-22.
AG151 cdc-25.1(nr2036)/dpy-5(e61) unc-13(e450) I. C. elegans Heterozygotes are WT and segregate WT, DpyUncs and adult steriles. Original deletion from Axys Pharmaceuticals.
AG152 unc-85(e1414) bli-2(e768) dpy-10(e128) II. C. elegans Unc and Dpy. Not Blistered: dpy-10 suppresses the appearance of the blisters.
AG165 san-1(av31) unc-13(e450) I. C. elegans Unc. Reduced brood size. Larval lethal, larval arrest. Steriles. Bursts at vulva. Can be maintained as a homozygote.
AG166 mdf-2(av16) unc-17(e245) IV. C. elegans Reduced brood size. Reduced hatching. Slow growth. Larval lethal. Larval arrest. Bursts at vulva. Suppresses the mat-3 one-cell arrest at 25C.
AG168 fzy-1(av15) unc-4(e120) II. C. elegans Unc. Gain-of-function allele of fzy-1.
AG171 mdf-1(av19) unc-42(e270) V. C. elegans Unc. Previously called AG164 by the CGC.
AG226 rol-6(e187) unc-4 (e120)/mnC1 [dpy-10(e128) unc-52(e444) nIs190 let-?] II; him-8(e1489) IV. C. elegans nIs190 [myo-2::GFP]. Him. Heterozygotes are wild-type GFP+ and segregate WT GFP+ heterozygotes, Rol Uncs, dead embryos, and males. nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1.
AG247 tyms-1(tm2429) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP tm2429 homozygotes (embryonic lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Reference: Jaramillo-Lambert A, et al. G3 (2015).
AG248 mus-101(tm1761) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP tm1761 homozygotes (embryonic lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Reference: Jaramillo-Lambert A, et al. G3 (2015).
AG50 daf-7(e1372) dpy-1(e1) III. C. elegans Maintain at 15C. Temperature-sensitive dauer constitutive: 100% dauers at 25C, leaky at 20C. Dpy. Crowds.
COP262 unc-119(ed3) III; knuSi221. C. elegans knuSi221 [fib-1p::fib-1(genomic)::eGFP::fib-1 3' UTR + unc-119(+)]. Single copy insertion. fib-1 promoter, genomic sequence, and 3'UTR was inserted into pCFJ151 (ttTi5606) targeting vector and inserted into ttTi5605. Allen AK, Nesmith JE, and A Golden. 2014 G3:4(12)2329-43.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
av31 Allele
av16 Allele
av15 Allele
av19 Allele substitution