Laboratory Information

NameAD View on WormBase
Allele designationas
HeadAndrew W Singson
InstitutionWaksman Institute, Piscataway, NJ
Address 190 Frelinghuysen Rd.
Piscataway 08854
United States
Website http://mbclserver.rutgers.edu/labs/singson/
Gene classes egg 

Strains contributed by this laboratory

Strain Genotype Species Description
AD186 egg-1(tm1071) III. C. elegans Temperature sensitive sterile. Maintain at 20C. Fertility is <10% of WT at 25C.
AD189 unc-119(ed3) III; asIs2. C. elegans asIs2 [pie-1p::GFP::egg-1 + unc-119(+)]. Oocyte membranes are GFP+.
AD200 unc-119(ed3) III; asIs1. C. elegans asIs1 [pie-1p::GFP::egg-3 + unc-119(+)].
AD213 spe-19(eb52) V; asEx83. C. elegans asEx83 [spe-19(+) + myo3p::GFP]. Pick GFP+ to maintain. asEx83 contains 7.3kb genomic fragment including spe-19 (Y113G7A.10) and 850bp of upstream sequence. Transgene rescues spe-19(eb52) sperm activation defect. GFP+ hermaphrodites are fertile. non-GFP hermaphrodites are sterile. All males are fertile.
AD226 egg-3(tm1191)/mIn1 [mIs14 dpy-10(e128)] II. C. elegans Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP tm1191 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain.
AD238 asIs2. C. elegans asIs2 [pie-1p::mCherry::egg-3].
AD265 nnIs2. C. elegans nnIs2 [pie-1p::GFP::chs-1 + unc-119(+)].
AD266 egg-4(tm1508) egg-5(ok1781) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1508 ok1781 homozygotes (maternal sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Parry JM, et al 2009 Current Biology 19(20):1752-7.
AD271 spe-38(eb44) I; him-5(e1490) V; asEx78. C. elegans asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile.
AD281 spe-45(as38) IV; him-5(e1490) V. C. elegans Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055
RT495 unc-119(ed3) III; asIs4. C. elegans asIs4 [egg-2::GFP + unc-119(+)]. Oocyte membranes are green.
RT497 unc-119(ed3) III; asIs3. C. elegans asIs3 [egg-1::GFP + unc-119(+)]. Oocyte membranes are green.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
as38 Allele substitution nonsense