| AD186 |
egg-1(tm1071) III. |
C. elegans |
Temperature sensitive sterile. Maintain at 20C. Fertility is <10% of WT at 25C. |
| AD189 |
unc-119(ed3) III; asIs2. |
C. elegans |
asIs2 [pie-1p::GFP::egg-1 + unc-119(+)]. Oocyte membranes are GFP+. |
| AD200 |
unc-119(ed3) III; asIs1. |
C. elegans |
asIs1 [pie-1p::GFP::egg-3 + unc-119(+)]. |
| AD213 |
spe-19(eb52) V; asEx83. |
C. elegans |
asEx83 [spe-19(+) + myo-3p::GFP]. Pick GFP+ to maintain. asEx83 contains 7.3kb genomic fragment including spe-19 (Y113G7A.10) and 850bp of upstream sequence. Transgene rescues spe-19(eb52) sperm activation defect. GFP+ hermaphrodites are fertile. non-GFP hermaphrodites are sterile. All males are fertile. |
| AD226 |
egg-3(tm1191)/mIn1 [mIs14 dpy-10(e128)] II. |
C. elegans |
Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP tm1191 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. |
| AD238 |
asIs2. |
C. elegans |
asIs2 [pie-1p::mCherry::egg-3]. |
| AD265 |
nnIs2. |
C. elegans |
nnIs2 [pie-1p::GFP::chs-1 + unc-119(+)]. |
| AD266 |
egg-4(tm1508) egg-5(ok1781) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
C. elegans |
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1508 ok1781 homozygotes (maternal sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Parry JM, et al 2009 Current Biology 19(20):1752-7. |
| AD271 |
spe-38(eb44) I; him-5(e1490) V; asEx78. |
C. elegans |
asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile. |
| AD281 |
spe-45(as38) IV; him-5(e1490) V. |
C. elegans |
Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055 |
| AD309 |
spe-21(syb4299) III; asEx98. |
C. elegans |
asEx98 [spe-21(+) + myo-3::GFP]. Pick GFP+ animals to maintain. syb(4299) is a non-conditional allele of spe-21. Mutant hermaphrodites and males are severly subfertile. Array carries a fragment (PCR product) of R13F6.5 containing a wild-type copy spe-21(+). spe-21/R13F6.5 formerly known as dhhc-5. The extrachromosomal array effectively rescues the fertility defect. Reference: Suryanarayanan S, et al. bioRxiv 2025.03.03.641263; doi: https://doi.org/10.1101/2025.03.03.641263. |
| AD316 |
spe-21(syb4179[spe-21::mNG]) III. |
C. elegans |
mNeonGreen tag inserted at the C-terminus of the endogenous spe-21 locus by CRISPR. Expression in the membranous organelles of sperm. Reference: Suryanarayanan S, et al. bioRxiv 2025.03.03.641263; doi: https://doi.org/10.1101/2025.03.03.641263. |
| AD319 |
spe-38(syb6556[spe-38::wrmScarlet-I]) I; him-5(e1490) V. |
C. elegans |
wrmScarlet-I tag inserted into endogenous spe-38 locus. Him. wrmScarlet-I expression labels membranous organelles (MOs) in the sperm. Reference: Zuo Y, et al. Biomolecules. 2023; 13(4):623. https://doi.org/10.3390/biom13040623 |
| RT495 |
unc-119(ed3) III; asIs4. |
C. elegans |
asIs4 [egg-2::GFP + unc-119(+)]. Oocyte membranes are green. |
| RT497 |
unc-119(ed3) III; asIs3. |
C. elegans |
asIs3 [egg-1::GFP + unc-119(+)]. Oocyte membranes are green. |
