Variation Information: cxTi9279

NamecxTi9279 View on WormBase
Species C. elegans
Genetic positionII:0.75 +/- 0.000 cM
Genomic positionII: 8219098..8219098
Protein changeM110.4a M110.4b M110.4c M110.4d M110.4e Insertion

Strains carrying this variation

Strain Genotype Species Description
KX54 ifg-1(cxTi9279) II; bcIs39 V. C. elegans bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Temperature-sensitive germ cell apoptosis leading to infertility at 25 C. Apoptosing germ cells are decorated by CED-1::GFP in the gonad. Loss of p170 form of IFG-1 (but not p130 IFG-1) confirmed by Western blot. Escaping eggs are fertilized and embryonic lethal. Mos-1 transposon insertion previously described as ifg-1::mos-1(cxP9279) II; confirmed by triple primer PCR with 5’-ACCAAACTGGGCAAACAAAG-3’, 5’-GCTCAATTCGCGCCAAACTATG-3’, and 5’-CTTCCTGAAATTTGGTTTAACAGT-3’. Homozygous ifg-1::mos yields only 444 bp product. Outcrossed heterozygotes yield both 353 bp (wild type ifg-1) and 444 bp products.