Variation Information: gk3507

Namegk3507 View on WormBase
Species C. elegans
Genetic positionV:-5.18 +/- 0.037 cM
Genomic positionV: 4502331..4502958
Protein changeT28F12.2a T28F12.2b T28F12.2c T28F12.2c T28F12.2d T28F12.2d T28F12.2d T28F12.2d T28F12.2d T28F12.2d T28F12.2d T28F12.2e T28F12.2f T28F12.2g T28F12.2h Deletion

Strains carrying this variation

Strain Genotype Species Description
VC3285 unc-62(gk3507) V/nT1 [qIs51] (IV;V). C. elegans T28F12.2. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk3507 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CGAAGCCAACACAAGAATCA. External right primer: TCGGTGTGCAAATCCAATTA. Internal left primer: ATCATCTTGCCGAAATCTGG. Internal right primer: TTTGACGTTCAGTTTGCTGG. Internal WT amplicon: 2229 bp. Deletion size: 628 bp. Deletion left flank: AGGCTTCCAATTTATTTTTTACACGCTTTT. Deletion right flank: GCGATAAATTTATCTGCAGGTCCTTTGAAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807