Variation Information: ok3268

Nameok3268 View on WormBase
Species C. elegans
Genetic positionI:-0.16 +/- 0.005 cM
Genomic positionI: 5286144..5286671
Protein changeC01G8.5a C01G8.5b Deletion

Strains carrying this variation

Strain Genotype Species Description
VC2616 erm-1(ok3268)/szT1 [lon-2(e678)] I; +/szT1 X. C. elegans C01G8.5. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3268 homozygotes. Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTTGAGTGTGTTGTTGCGA. External right primer: GCGCACATCCTTTTTCATTT. Internal left primer: ACAATCAGGGATTCCGTTTT. Internal right primer: TGGATGGAACATTTTGTGGA. Internal WT amplicon: 1263 bp. Deletion size: 528 bp. Deletion left flank: TTGTCTTGTAACAGTAGTCTGTCTCTCGAC. Deletion right flank: GTTTGTTGATACGGAGTCGTGGAGCGTAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807