Variation Information: ok2084

Nameok2084 View on WormBase
Species C. elegans
Genetic positionII:-0.22 +/- 0.003 cM
Genomic positionII: 6489051..6490543
Protein changeT28D9.10a T28D9.10b T28D9.2a T28D9.2b T28D9.2b T28D9.2b T28D9.2b Deletion

Strains carrying this variation

Strain Genotype Species Description
VC2013 snr-3&rsp-5(ok2084)/mIn1 [mIs14 dpy-10(e128)] II. C. elegans T28D9.10, T28D9.2. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok2084 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: GGTTCCGCAAAGTGCATAAT. External right primer: CTAGGAAGAGCGCGAACAAC. Internal left primer: GTTGACTCGGAAAGCCGTAA. Internal right primer: AGGAAGCGGTGTCCTACTCA. Internal WT amplicon: 3125 bp. Deletion size: 1493 bp. Deletion left flank: AGTATAGGACGTTCGATACTCAAATTTGCT. Deletion right flank: CGTGATCGCAAACGTTCTCGCAGATCCCGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807