Variation Information: gk3330

Namegk3330 View on WormBase
Species C. elegans
Genetic positionX:-19.50 +/- 0.005 cM
Genomic positionX: 822145..822405
Protein changeF25E2.5a F25E2.5b F25E2.5c F25E2.5d F25E2.5e F25E2.5f F25E2.5g F25E2.5h Definition Deletion

Strains carrying this variation

Strain Genotype Species Description
VC1985 F47G4.6(gk1056) I; daf-3(gk3330) X. C. elegans This strain is homozygous for a deletion (gk1056) in F47G4.6, detectable by PCR using the following primers. External left primer: CGCTTCTCCTGAGGTAGTGG. External right primer: GGACACTTCGAACCGGATTA. Internal left primer: ACGATGGATCGGTGTTTCTC. Internal right primer: AGCTGCCTAGCCTTCTCCTC. Internal WT amplicon: 1914 bp. Deletion size: 457 bp. Deletion left flank: TTAGCCTAAAAAATTTTTCCGAATTTTCTC. Deletion right flank: AGCTACCGTACTCATAAGCTACAGAGTGTA. Validation: gk1056 passed by diagnostic PCR and CGH. Other deletion (gk3330) identified by CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807