Variation Information: ok1658

Nameok1658 View on WormBase
Species C. elegans
Genetic positionI:-1.73 +/- 0.009 cM
Genomic positionI: 4040817..4042090
Protein changeC09D1.1a C09D1.1b C09D1.1e C09D1.1f C09D1.1k C09D1.1l C09D1.1m C09D1.1n C09D1.1o Deletion

Strains carrying this variation

Strain Genotype Species Description
VC1201 unc-89(ok1658) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans C09D1.1. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1658 homozygotes (probable early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TCAAGTTCTTTTCGGGTTGG. External right primer: AGCGAAAGAGCAGCATGATT. Internal left primer: TCAAACAGCGCATGAAAAAC. Internal right primer: TACCCAAAAACGGAAAATCG. Internal WT amplicon: 2637 bp. Deletion size: 1274 bp. Deletion left flank: TCCTATCATCTATTTCATTCGATCAAACAA. Deletion right flank: ATTTTGGGGGGGGGGGGGGGCAGAAATCGG. Breakpoints should be confirmed; deletion may also involve insertion and/or rearrangement of sequence between external left and internal left primers. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807