Variation Information: ok1656

Nameok1656 View on WormBase
Species C. elegans
Genetic positionI:5.03 +/- 0.009 cM
Genomic positionI: 10433248..10435185
Protein changeY106G6H.2 Y106G6H.2 Y106G6H.2 Deletion

Strains carrying this variation

Strain Genotype Species Description
VC1191 pab-1(ok1656) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Y106G6H.2. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1656 homozygotes (probable early larvarl arrest ). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TTCGTTTGCGATTCATTTCA. External right primer: CTAGACGTCGCCTGACTTCC. Internal left primer: GTTCAACATGTGTTGGTCCG. Internal right primer: GACCCAACTCCTCACCCATA. Internal WT amplicon: 2732 bp. Deletion size: 1938 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807