AMH3 |
unc-33(mn407) IV; sosIs5. |
C. elegans |
sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc. |
AMH32 |
juIs76 II; unc-33(mn407) IV. |
C. elegans |
juIs76 [unc-25p::GFP + lin-15(+)] II. |
AMH36 |
juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. |
C. elegans |
juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C. |
AMH57 |
unc-33(mn407); olaEx3013. |
C. elegans |
olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 |
AMH69 |
unc-33(mn407) IV; daf-2(e1370) III. |
C. elegans |
Maintain at 15C. Synthetic Lethality at 25C. |
SP1382 |
unc-33(mn407) IV. |
C. elegans |
|