NL3321 |
sid-1(pk3321) V. |
C. elegans |
Resistant to feeding dsRNA but sensitive to dsRNA injection. PKA rsd-8. |
TU3568 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. |
C. elegans |
uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
TU3595 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. |
C. elegans |
uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette. |
TU3596 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. |
C. elegans |
Him. Enhanced RNAi background. Maintain under normal conditions. |