MT7386 |
ced-9(n2812) III; ced-3(n717) IV. |
C. elegans |
n2812 is a strong loss-of-function allele and a maternal effect lethal. |
MT7686 |
ced-9(n2812)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT. Segregates Dpy Steriles. |
MT7735 |
ced-9(n2812) III; ced-3(n2427) IV. |
C. elegans |
|
PD9927 |
ced-9(n2812) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); nIs106 X. |
C. elegans |
nIs106 [lin-11::GFP + lin-15(+)] X. Homozygous maternal effect lethal ced-9 mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ced-9 homozygotes (maternal effect lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. |