Caenorhabditis Genetics Center (CGC)

Variation Information: ku298

Nameku298 View on WormBase
Species C. elegans
Genetic positionII:1.62 +/- 0.000 cM
Genomic positionII: 9641791..9641791
Protein changeZK1307.6a ZK1307.6b Substitution

Strains carrying this variation

Strain Genotype Species Description
MH1829 fzr-1(ku298) unc-4(e120) II. C. elegans Animals are healthy and Unc. Synthetic lethal/hyperproliferation with lin-35.
SV1448 fzr-1(ku298) II; unc-119(ed3) III; heSi143 IV; heSi141 X. C. elegans heSi 143 [rps-27::loxP::mCherry::let-858::fzr-1p::fzr-1::fzr-1 UTR::loxP::GFP::let-858 UTR + unc-119(+)] IV. heSi141 [hlh-8(short)::FLAG::CRE::tbb-2 + unc-119(+)] X. Maintain at 15-25C. Expression of CRE recombinase in the mesoblast lineage driven by the hlh-8 promoter. heSi143 rescues fzr-1(ku298). fzr-1 will be excised upon mesoblast-specific expression of CRE, creating a mesoblast specific mutant of fzr-1. This recombination event can be visualized by a switch from red to green in those cells (the mesoblast) were fzr-1 is lost. Reference: Ruijtenberg S & van den Heuvel S. Cell. 2015 Jul 16;162(2):300-13.