Caenorhabditis Genetics Center (CGC)

Variation Information: ok98

Nameok98 View on WormBase
Species C. elegans
Genetic positionIV:-6.77 +/- 0.012 cM
Genomic positionIV: 2641641..2643587
Protein changeY69A2AR.2a Y69A2AR.2b Y69A2AR.2c Y69A2AR.2d Y69A2AR.2d Y69A2AR.3 Deletion

Strains carrying this variation

Strain Genotype Species Description
KG115 lin-1(e1275) unc-33(e204)/ric-8(ok98) IV. C. elegans ok98/+ animals are slightly Egl-d and have a small vulval bump. ok98 homozygotes are straight and paralyzed and slow growing. About 2/3 eventually reach adulthood and are paralyzed and sterile (they produce few if any oocytes). Segregates Unc Muv(ts).
VC3138 ric-8(ok98) IV/nT1 [qIs51] (IV;V). C. elegans Y69A2AR.2. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok98 homozygotes (paralyzed, sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GTGTCTTTACATCCGTCATTTCTG. External right primer: CATGATCAATAGCCTTCACATCTC. Internal left primer: AAGCGTCCAAGGCACATATCG. Internal right primer: CGTCTTCAACGCCTCGGTAG. Internal WT amplicon: 3370 bp. Deletion size: approximately 1480 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807