| CF1814 |
rrf-3(pk1426) II; daf-2(e1370) III. |
C. elegans |
Daf-c at 25.5C; grow at 20C or less. Long lived. |
| CF1980 |
rrf-3(pk1426) II; daf-2(e1368) III. |
C. elegans |
Daf-c at 25.5C; grow at 20C or less. Long lived. |
| IU10 |
daf-16(mgDf47) I; rrf-3(pk1426) II. |
C. elegans |
|
| IU7 |
rrf-3(pk1426) II; sir-2.1(ok434) IV. |
C. elegans |
|
| JU2058 |
rrf-3(pk1426) II; mfIs70 IV; rde-1(ne219) V. |
C. elegans |
mfIs70 [lin-31p::rde-1 + myo2p::GFP]. mfIs70 is a spontaneous integration in LG IV. Superficially wild-type. This strain can be used for Pn.p-specific RNAi. Reference: Barkoulas et al. (2013) Developmental Cell Jan 14;24(1):64-75 |
| LH191 |
lrp-1(ku156) eqIs1 I; rrf-3(pk1426) II. |
C. elegans |
eqIs1 [lrp-1::GFP] I. eqIs1 is a spontaneous insertion of an lrp-1::GFP transgene extremely close to the endogenous lrp-1 gene that completely rescues lrp-1(ku156) Mlt and larval lethality. Reference: Kang YL, et al. Mol Biol Cell. 2013 Feb;24(3):308-18. |
| NK1339 |
rrf-3(pk1426) II; qyIs127 V; qyIs166 X. |
C. elegans |
qyIs127 [lam-1p::lam-1::mCherry + unc-119(+)] V. qyIs166 [cdh-3p::GFP::CAAX + unc-119(+)] X. Temperature-sensitive sterile; maintain at 20C or lower for optimum fertility. Increased sensitivity to RNAi when compared to wild-type animals. lam-1p::lam-1::mCherry expression can be weak and variable. Reference: Kelley, LC, et al. Developmental Cell. 2019 Feb 11;48(3):313-328.e8. |
| NK2115 |
cpIs121 I; rrf-3(pk1426) II; rde-1(ne219) V. |
C. elegans |
cpIs121 [lag-2p::mNG::PH::F2A::rde-1] I. Temperature-sensitive: maintain at 16-20C. RNAi-response variant. RNAi-hypersensitized DTC-specific RNAi strain that labels all rde-1(+) cells with mNeonGreen. Reference: Linden LM, et al. Dev Biol. 2017 Sep 1;429(1):271-284. |
| NK640 |
rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs102. |
C. elegans |
qyIs102 [fos-1ap::rde-1(genomic) + myo-2::YFP + unc-119(+)]. Uterine-specific RNAi. |
| NK741 |
rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs138. |
C. elegans |
qyIs138 [unc-62p::rde-1(genomic) + myo-2::YFP + unc-119(+)]. VPCs sensitive to RNAi. Vul phenotype from lin-39 RNAi depletion. |
| NK742 |
rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs139. |
C. elegans |
qyIs139 [unc-62p::rde-1(genomic) + myo-2::YFP + unc-119(+)]. VPCs sensitive to RNAi. Vul phenotype from lin-39 RNAi depletion. |
| NL2099 |
rrf-3(pk1426) II. |
C. elegans |
Homozygous rrf-3 deletion allele. Increased sensitivity to RNAi when compared to WT animals. Deletion sequence (deletion in lower case letters, flanking undeleted sequence in capital letters): TGCACATATTctacagaatt ------- --------tacccgattaAATGGACAATT (from Plasterk Lab 11/05). |