CB2620 |
daf-9(e1406)/lon-2(e678) X. |
C. elegans |
Heterozygotes are WT and segregate WT, lethal dauers (DAUER-LIKE LARVAE) and Lon. Can recombine; check for correct segregation of progeny to maintain. |
DR733 |
lon-2(e678) daf-9(e1406)/unc-78(e1217) X. |
C. elegans |
Balanced lethal dauer constitutive. Heterozygotes are WT and segregate WT, LonDaf and Unc. Will get 3-5 adult Lon per clone. Maintain by picking WT. |
DR921 |
dpy-7(sc27) daf-9(e1406)/lon-2(e678) X. |
C. elegans |
Heterozygotes are WT. At 25C the hets segregate DpyDafs, WT and Lon. At 15C the hets segregate Dafs, WT and Lon. [dpy-7(sc27) is temperature sensitive. At 25C it is larger than most Dpys and has a tendency to roll. Not expressed at 15C.] Maintain by picking WT. |
GR2118 |
daf-9(e1406) dpy-7(sc27) X; mgEx663. |
C. elegans |
mgEx663 [dpy-7p::daf-9B(cDNA)::GFP + mec-7::GFP]. Pick GFP+ to maintain. GFP fusion containing 0.4 kb dpy-7 promoter and daf-9 isoform B cDNA. Reference: Mak HY, Ruvkun G. Development. 2004 Apr;131(8):1777-86. |