Variation Information: e12

Namee12 View on WormBase
Species C. elegans
Genetic positionIV:-26.77 +/- 0.000 cM
Genomic positionIV: 258714..258714
Protein changeT21D12.2a T21D12.2b Substitution

Strains carrying this variation

Strain Genotype Species Description
CB12 dpy-9(e12) IV. C. elegans Dpy.
MT4954 dpy-9(e12) ced-2(e1752) unc-33(e204) IV. C. elegans Dpy. Unc. Abnormal Cell Death.
MT6025 lin-31(n301) II; dpy-9(e12) IV; unc-51(e369) V. C. elegans Mapping strain.
MT628 dpy-9(e12) unc-17(e245) IV. C. elegans Dpy. Unc.
MT6996 dpy-9(e12) ced-2(e1752) lin-1(e1275) IV. C. elegans Dpy. Temperature sensitive Muv. Abnormal cell death.
RG3131 ears-2(ve631[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) /dpy-9(e12) IV. C. elegans Homozygous sterile, Pvl. Deletion of 1629 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, GFP+ non-Dpy Pvl sterile adults (ve631 homozygotes), Dpy non-GFP (dpy-9(e12) homozygotes). Maintain by picking wild-type GFP+. Left flanking Sequence: acaatggaaatcggagTTATAATAATTCCT ; Right flanking sequence: CGGTTAGCTTCATaatttctattgaggggt. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.
RW3568 pat-6(st561)/dpy-9(e12) IV. C. elegans Heterozygotes are WT and segregate WT, Dpys and Pats. Maintain by picking WT and checking for correct segregation of progeny.
VC1180 gex-2(ok1603)/dpy-9(e12) IV. C. elegans F56A11.1. Homozygous lethal deletion chromosome balanced by morphological marker. Heterozygotes are WT, and segregate WT, Dpy (dpy-9 homozygotes), and ok1603 homozygotes (probably sterile adult with protruding or ruptured vulva). Pick WT and check for correct segregation of progeny to maintain. External left primer: AGTCACCCTCTCTCCCACCT. External right primer: AAGCAGTGGGTTGAAAAACG. Internal left primer: CAGCTGAAAAATGAACGCAA. Internal right primer: GTGTTGGCCGCTGTATCTTT. Internal WT amplicon: 2748 bp. Deletion size: 1903 bp. Deletion left flank: AAACATACCAGATGTTTTGCAGCTTCTCGA. Deletion right flank: GCAAATTTGGCAAATTTGCCGAGCTCGGCA. Insertion Sequence: ATTTGGCAAATTTGGCAAATTTGCCGGCAAATTTGGCAAATTTGCCTA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC137 R02D3.5(ok269)/dpy-9(e12) IV. C. elegans R02D3.5. Heterozygotes are WT and segregate WT, Dpy-9 and grotty sterile adults with vulval blip (ok269 homozygotes). Pick WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807