Variation Information: e1147

Namee1147 View on WormBase
Species C. elegans
Genetic positionIV:3.27 +/- 0.000 cM
Genomic positionIV: 6977571..6977571
Protein changeZK381.1a Substitution

Strains carrying this variation

Strain Genotype Species Description
GE2940 unc-32(e189) pnm-2(t1445)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2941 unc-32(e189) let-(t1446)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles and Uncs which give only dead eggs. This strain was mistakenly called emb-30 in the paper; it is not an emb-30 allele.
GE2946 let-748(t1452) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2948 unc-32(e189) apo-2(t1454)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2952 unc-32(e189) pnm-3(t1458)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE2959 unc-32(e189) tbg-1(t1465)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tbg-1(t1465) previously called sas-3(t1465).
GE2961 unc-32(e189) csg-4(t1467)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE3023 emb-8(t1533) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
GE3633 unc-32(e189) cyk-4(t1689)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs.
JH2874 pgl-1(ct131) him-3(e1147) III; ozIs5. C. elegans ozIs5 [gld-1::GFP + unc-119(+)].
JH2877 fbf-2(q738) II; pgl-1(ct131) him-3(e1147) III; axIs1722. C. elegans axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background.
JH2883 fbf-1(ok91) II; pgl-1(ct131) him-3(e1147) III; axIs1722. C. elegans axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background.
MG8 csc-1(t1171) dpy-2(e8)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpyUncs, and Dpys which give only dead eggs. Throws males.
SS2 pgl-1(ct131) him-3(e1147) IV. C. elegans Temperature sensitive sterility. The sterility has both a maternal and a non-maternal component. Can be maintained indefinitely at low temperatures (16-23 C), with 7-19% sterile offspring. When low-temperature-grown homozygotes are allowed to produce progeny at 25C, the percentage of sterile offspring is 75-85%; at 26C the percentage of sterile offspring is 100%. Throws males.
SS617 pgl-1(ct131) him-3(e1147) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. C. elegans Throws males. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS618 pgl-1(ct131) him-3(e1147) IV; pgl-3(bn104) V. C. elegans Throws males. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS729 pgl-2(bn123) III; pgl-1(ct131) him-3(e1147) IV. C. elegans Throws males. Maintain at 15C. Sterile at higher temperatures.
TX773 teIs65 II; unc-119(ed3) III; him-3(e1147) IV. C. elegans teIs65 [pie-1p::GFP::plk-1(PBD) + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs65 contains GFP fused to the PLK-1 protein-binding domain. Derived from injection of pRL1216. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97.
TX796 unc-119(ed3) III; him-3(e1147) IV; teEx321. C. elegans teEx321[pRL1636 (Pmed-1::GFP::sys-1) pDPmm016]. GFP signal is very weak and can't be see using a dissecting microscope. Very low transmission. Most signal is nuclear and the signal is stronger in the posterior sister, e.g., MSp stronger than MSa, Ep stronger than Ea. Some cortical signal was also detected. Some larvae lack anterior gut or have gaps. Maintain by picking non-Uncs.
TX895 unc-119(ed3) III; him-3(e1147) IV; teIs84 X. C. elegans teIs84 [end-3p::GFP::H2B + unc-119(+)] X. Him. Superficially wild-type. Integrated E-lineage specific GFP reporter. Reference: Robertson SM, et al. PLoS One. 2014 Sep 2;9(9):e106309.
TX903 teIs90 I; unc-119(ed3) III; him-3(e1147) IV. C. elegans teIs90 [(pRL1483) pie-1p::GFP::taf-4 + unc119(+)]. Sick at high temperatures. Maintain at 20C, but it will silence after some generations.
TX964 unc-119(ed3) III; him-3(e1147) IV; teIs98. C. elegans teIs98 [(pRL1450) pie-1p::GFP::sys-1 + unc-119(+)].