Variation Information: e53

Namee53 View on WormBase
Species C. elegans
Genetic positionIV:1.76 +/- 0.000 cM
Genomic positionIV: 5499260..5499260
Protein changeB0273.4a B0273.4b B0273.4c B0273.4d B0273.4d B0273.4e B0273.4f Substitution

Strains carrying this variation

Strain Genotype Species Description
CB1039 unc-5(e53) IV; nuc-1(e1392) X. C. elegans Unc. DNAse undetectable. Gut DNA fluoresence abnormal. M-MATING-NO SUCCESS.
CB3695 fem-1(e1965)/unc-5(e53) mor-2(e1125) IV. C. elegans WT heterozygote, fem-1(e1965) maternal. Segregates WT heterozygotes, WT fem-1 homozygotes that give only fertile female progeny, and UncMor. (Probably has lost mor-2 [Edgley, 4/92]. When confirmed, should be given a new strain name.)
CB4457 fem-1(e2342) / unc-5(e53) mor-2(e1125) IV. C. elegans Wild-type hermaphrodites segregating WT, Fem, Unc Mor. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.
CB4760 fem-1(e2044) mor-2(e1125) unc-24(e158) fem-3(q20) / unc-5(e53) dpy-20(e1282) IV C. elegans Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.
CGC11 unc-5(e53)/nT1 [umnIs1] IV; dpy-11(e224)/nT1 V. C. elegans umnIs1 [eft-3p::GFP + HygroR, V:~2821000] V. umnIs1 GFP is expressed at low levels, and might be difficult to see in heterozygotes. Heterozygotes are WT and segregate WT, DpyUnc, Vul and dead eggs. Maintain by picking GFP+ WT. Derived by insertion of GFP transgene into parental strain MT1000 using MosSCI.
CGC13 unc-5(e53)/nT1 [umnIs3] IV; dpy-11(e224)/nT1 V. C. elegans umnIs3 [eft-3p::NLS::tdTomato + HygroR, V:~2821000] IV. tdTomato is expressed at low levels, and might be difficult to see in heterozygotes. Heterozygotes are WT and segregate WT, DpyUnc, Vul and dead eggs. Maintain by picking WT with tdTomato expression. Derived by insertion of tdTomato transgene into parental strain MT1000 using CRISPR/Cas9.
CGC33 unc-5(e53)/nT1 [umnIs22] IV; dpy-11(e224)/nT1 V. C. elegans umnIs22 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] IV. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, Vul GFP+ (nT1) and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9.
CGC39 unc-5(e53)/nT1 IV; dpy-11(e224)/nT1 [umnIs28] V. C. elegans umnIs28 [myo-2p::GFP + NeoR, IV: 12457861 (intergenic)] V. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, Vul GFP+ (nT1) and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9.
CGC63 unc-5(e53)/nT1 [umnIs49] IV; dpy-11(e224)/nT1 V. C. elegans umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, Vul mKate2+ (nT1) and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9.
CGC71 unc-5(e53)/nT1 IV; dpy-11(e224)/nT1 [umnIs57] V. C. elegans umnIs57 [myo-2p::mKate2 + NeoR, IV: 12457861 (intergenic)] V. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, Vul mKate2+ (nT1) and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9.
DR169 che-3(e1378) I; unc-5(e53) IV. C. elegans Dauer defective. Unc-coiler.
DR170 unc-5(e53) IV; che-2(e1033) X. C. elegans Dauer defective. Unc-coiler.
DR640 ama-1(m118) unc-5(e53) IV. C. elegans Hypercontracted and amanitin resistant.
DR690 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-272(m243) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc, and DpyLet.
DR691 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-287(m244) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLets are sterile adults. Maintain by picking semi-Dpy.
DR692 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-275(m245) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLets. Lethal mid-larval. Maintain by picking semi-Dpy.
DR694 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-281(m247) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLets are adult steriles. Maintain by picking semi-Dpy.
DR695 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-285(m248) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLet are adult steriles. Maintain by picking semi-Dpy.
DR703 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-274(m256) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc, and DpyLets. Lethal early larval. Maintain by picking semi-Dpy.
DR705 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-282(m258) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. Lethal early larval. Maintain by picking semi-Dpy.
DR706 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-280(m259) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLets. The DpyLets are adult steriles. Maintain by picking semi-Dpy.
DR709 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-279(m261) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLets. The DpyLets are adult steriles. Maintain by picking semi-Dpy.
DR710 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-277(m262) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpys, Uncs and Lets. Lethal mid-larval. Maintain by picking semi-Dpy.
DR711 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-273(m263) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. Lethal early larval. Maintain by picking semi-Dpy.
DR715 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-284(m267) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. Lethal early larval. Maintain by picking semi-Dpy.
DR717 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-286(m269) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLet are adults which lay eggs that do not hatch. Maintain by picking semi-Dpy.
DR767 unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-288(m306) IV. C. elegans Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc, and DpyLets. DpyLets are adult steriles. Maintain by picking semi-Dpy.
EU84 unc-5(e53) skn-1(zu67) IV/nT1 [let-?(m435)] (IV;V). C. elegans Heterozygotes are WT and segregate WT, Uncs which give only dead eggs, and dead eggs.
JK3087 ehn-3(q689) unc-5(e53) IV. C. elegans Unc. About 20% have single gonad arm. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK551 unc-5(e53) fem-3(q22) IV. C. elegans Temperature sensitive. At 25C, XX germline makes only sperm; at 15C, germline makes oocytes and excess sperm. Unc. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
MT1000 unc-5(e53)/nT1 IV; dpy-11(e224)/nT1 V. C. elegans Heterozygotes are WT and segregate WT, DpyUnc, Vul and dead eggs. Maintain by picking WT.
MT2867 unc-36(e251) III; unc-5(e53) IV; dpy-11(e224) V; lon-2(e678) lin-15B&lin-15A(n765) X. C. elegans
MT3918 unc-5(e53) sem-3(n1655) IV. C. elegans
MT464 unc-5(e53) IV; dpy-11(e224) V; lon-2(e678) X. C. elegans Mapping strain. DpyUnc.
MT4867 unc-5(e53) lin-45(n2018) IV. C. elegans Unc. n2018 is cold sensitive. Most animals at 15C are Vul/Let. AT 25C, 25% of the animals are non-Vul.
MT5240 unc-5(e53) lin-33(n1043) bli-6(sc16) IV. C. elegans Unc. Vul. Blistered.
MT5241 unc-5(e53) unc-44(e362) IV. C. elegans Unc.
MT5242 unc-5(e53) bli-6(sc16) IV. C. elegans Blistered Unc.
RU51 let-23(n1045) II; unc-5(e53) IV. C. elegans Unc and Vul.
RW3616 deb-1(st554)/unc-5(e53) unc-24(e138) IV. C. elegans Heterozygotes are WT and segregate WT, Uncs and Pats. Maintain by picking WT and checking for correct segregation of progeny. Received new stock from Waterston lab 5/98. st554 previously called pat-8.
SP1052 dpy-13(e184) unc-5(e53) IV. C. elegans DpyUnc.
TH11 unc-5(e53) dpy-20(e1282) IV. C. elegans Unc. ts Dpy.
VC362 unc-5(e53) IV/nT1 [qIs51] (IV;V); dpy-11(e224) V/nT1 [qIs51] (IV;V). C. elegans Morphological markers unc-5 and dpy-11 balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested nT1 aneuploid progeny, and GFP- unc-5; dpy-11 homozygotes. nT1[qIs51] homozygotes inviable. Pick GFP+ WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
WM103 unc-5(e53) mbk-2(ne3442) IV. C. elegans Unc. Maintain at 15C. Produces dead eggs at 25C.
WU57 lin-45(n2510) unc-24(e138)/unc-5(e53) dpy-20(e1282) IV. C. elegans Heterozygotes are non-Unc and segregate non-Unc, Sterile Unc, and Dpy Unc. n2510 is a strong lin-45 raf allele: 100% of homozygotes are Sterile and Vul (no discernable vulva) or larval lethal.