| MT20108 |
dpy-17(e164) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] nIs281 III. |
C. elegans |
nIs281 [myo-2::RFP] integrated near qC1. Recombination between nIs281 and qC1 has been reported. Fails to complemement all markers on qC1. Heterozygotes are WT. Segregates Dpy Sterile and Dpy Unc. |
| MT20109 |
dpy-17(e164) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III. |
C. elegans |
nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1. Fails to complement all markers on qC1. Heterozygotes are WT GFP+. Segregates GFP+ Dpy Sterile and non-GFP Dpy Unc. |
| MT20113 |
unc-32(e189) dpy-18(e499)/eT1 III; +/eT1 nIs267 V. |
C. elegans |
nIs267 [myo-2::GFP] integrated in or near eT1. Heterozygotes are wild-type and segregate WT, Dpy Unc, and Unc. Maintain by picking wild-type; check for presence of Unc progeny. |
| MT2343 |
dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. |
C. elegans |
Heterozygotes are Muv and Egl (n137 is semi-dominant) and segregate DpyMuv and Unc lethals (most arrest as L1-L3, the few survivors are sterile, scrawny, Unc and have a large ventral blip at hte position of the vulva). Maintain by picking non-Dpy non-Unc. |
| MT2351 |
lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. |
C. elegans |
Heterozygotes are Muv. Segregates DpyMuv and Unc. |
| MT3022 |
nDf20/sma-2(e502) unc-32(e189) III. |
C. elegans |
Heterozygotes are WT and segregate WT, SmaUnc and dead eggs. Maintain by picking WT. |
| MT3025 |
let-23(n1045) II; lin-13(n387) unc-36(e251)/unc-32(e189) III. |
C. elegans |
Heterozygotes are Vulvaless and segregate more Vul, Vul Unc-36 and Vul Unc-32. n1045 is cold sensitive-strain gives some arrested larvae (L2) which look like little sticks. |
| MT3751 |
dpy-5(e61) I; rol-6(e187) II; unc-32(e189) III. |
C. elegans |
|
| MT4009 |
lin-39(n1760)/dpy-17(e164) unc-32(e189) III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain. |
| MT4500 |
lin-39(n1880)/dpy-17(e164) unc-32(e189) III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain. |
| MT465 |
dpy-5(e61) I; bli-2(e768) II; unc-32(e189) III. |
C. elegans |
Mapping strain. DpyUnc. |
| MT4947 |
ced-1(e1735) I; unc-32(e189) ced-7(n1892) III. |
C. elegans |
Cell corpses persist. Unc. n1892 is the strongest allele of ced-7. |
| MT4979 |
lon-1(e185) ced-6(n1813) unc-32(e189) ced-7(n1892) III. |
C. elegans |
ced-6(n1813): dead cells persist, maternal rescue of embryonic. Unc. Long. |
| MT4997 |
unc-32(e189) ced-7(n1892) III. |
C. elegans |
Unc. Persistent cell corpses-Maternal effect. |
| MT5000 |
unc-32(e189) ced-7(n1892) III; ced-10(n1993) IV. |
C. elegans |
Unc. |
| MT688 |
lin-12(n137)/unc-32(e189) III; him-5(e1467) V. |
C. elegans |
Heterozygotes are Muv and segregate Muv, Unc and males. Maintain by picking Muv which throw Uncs. |
| MT706 |
lin-13(n388)/unc-32(e189) III. |
C. elegans |
Maintain by picking wild-type animals raised at 25C. Heterozygotes will be wild-type and segregate wild-type, Unc, and Sterile Muv. The phenotype of homozygous lin-13 hermaphrodites segregating from a heterozygous mother depends on the temperature at which the strain was grown. At 25C, homozygous hermaphrodites segregating from a heterozygote are both Muv and sterile. At 20C, ~1/2 of hermaphrodites segregating from a heterozygote are sterile, but only a few are Muv. At 15C, hermaphrodites segregating from a heterozygote are almost wild type in appearance and fertility. However, if the progeny of these 15C animals are grown at 15C, all are sterile and some are Muv. If the progeny of these 15C animals are grown at 25C, then some animals arrest during larval growth and the rest are both sterile and Muv. Reference: Ferguson EL & Horvitz HR. Genetics. 1985 May;110(1):17-72. PMID: 3996896. |
| NG2618 |
yDf10 unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles and dead eggs. Derived from strain TY1353. Grows fairly slowly but seems more stable than TY1353, which gives lots of steriles. |
| OH2638 |
dpy-17(e164) let-756(s2887) unc-32(e189) III; oyIs14 V; otEx1467. |
C. elegans |
oyIs14 [sra-6::GFP + lin-15(+)]. otEx1467 [let-756(+) + ceh-22p::GFP]. Animals carrying otEx1467 are DpyUnc. Animals which have lost the otEx1467 array are DpyUncLet. |
| OH2639 |
dpy-17(e164) let-756(s2887) unc-32(e189) III; oyIs14 V; otEx1468. |
C. elegans |
oyIs14 [sra-6::GFP + lin-15(+)]. otEx1468 [let-756(+) + ceh-22p::GFP]. Animals carrying otEx1468 are DpyUnc. Animals which have lost the otEx1468 array are DpyUncLet. |
| PJ1153 |
clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V. |
C. elegans |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc. clr-1 is temperature-sensitive. |
| PJ1155 |
let-756(s2613) unc-32(e189) III; ccIs55 V; egl-17(n1377) X. |
C. elegans |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egg laying defective. Moderate to severe bloating. >50% make bags of worms. |
| PJ1158 |
clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V; egl-17(n1377) X. |
C. elegans |
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egl: moderate to severe bloating. Clr-1 is ts. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc. |
| PS2943 |
hmg-1.2(sy549) unc-32(e189) III; syIs20 him-5(e1490) V. |
C. elegans |
syIs20 [gpa-1::lacZ + dpy-20(+)] V. Unc. Him. p-Vul. Abnormal secondary vulva lineage defect in 10% of animals. Reduced brood size as well as other pleiotropic defects. Males have ray defect (cannot mate) and SPD sheath to neuron transformation, which can be visualized by lacZ staining in syIs20 [gpa-1::lacZ] background: 1 cell per spicule stains in WT (the SPD neuron) whereas in hmg-1.2(sy549), 2 cells per spicule stain. sy549 previously called son-1. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| RL67 |
lon-1(e185) let-711(it150) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles, and Lon Uncs which are temperature sensitive maternal effect lethals. Embryos from homozygyous it150 hermaphrodites have spindle orientation defects at second and third cleave at 25C. |
| RW1522 |
pat-2(st538) unc-32(e189) III; stEx10. |
C. elegans |
Animals with the extrachromosomal array are Unc Rollers. Animals which have lost the array are Pat. Strain can be propagated by chunking. The exact construct used to create stEx10 is unknown; it is thought to carry a pat-2 rescuing construct (most likely a cosmid) with a Rol marker. |
| SP17 |
unc-32(e189) dpy-1(e1) III. |
C. elegans |
DpyUnc. |
| SP462 |
dpy-1(e1) unc-36(e251) III; mnDp37[unc-32(e189)] (III;f). |
C. elegans |
Animals with the duplication are WT. Animals which have lost the duplication are DpyUnc-36. |
| SP471 |
dpy-17(e164) unc-32(e189) III. |
C. elegans |
|
| ST20 |
ncIs2 II; mup-4(nc20)/sma-3(e491) unc-32(e189) III. |
C. elegans |
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, SmaUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2. |
| ST21 |
ncIs2 II; mup-4(nc21)/dpy-17(e164) unc-32(e189) III. |
C. elegans |
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2. |
| ST22 |
ncIs2 II; mup-4(nc22)/dpy-17(e164) unc-32(e189) III. |
C. elegans |
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2. |
| ST35 |
ncIs2 II; mup-4(nc35)/dpy-17(e164) unc-32(e189) III. |
C. elegans |
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest as embyros or in larval development. Not well balanced. Neurons visualized with ncIs2. |
| SX340 |
unc-32(e189) mut-7(pk204) pha-1(e2123) III; ccEx7271. |
C. elegans |
ccEx7271 [let-858::GFP + pha-1(+)]. This strain expresses nuclear-localized GFP in all somatic nuclei, but reduced or no GFP in germ cells. If maintained at 20C, pha-1(ts) genotype will select for transgenic animals. Germ cell expression can be observed when maintained at 25C. Germline transgene silencing is abnormal. |
| TY1353 |
yDf10 unc-32(e189)/unc-93(e1500) dpy-17(e164) III. |
C. elegans |
Heterozygotes are Unc-93 and segregate more Unc-93, yDf10 homozygotes (dead eggs) and Unc-93 Dpy-17 homozygotes (young dpy-17 larvae are easily recognizable as abnormal spindle-shaped things). Difficult to maintain and use. yDf10 apparently causes semi-sterility (a second strain constructed by the Mark Edgley at the CGC, yDf10/qC1, exhibits same sterility), and unc-93 is Egl and difficult to mate into. Some Df homozygotes are laid, but most remain inside the mother. |
| TY415 |
unc-32(e189) dpy-28(s939) III/eT1 III; +/eT1 V. |
C. elegans |
WT strain which segregates WT, Unc-36, DpyUnc and dead eggs. DpyUncs give 6-10% viable progeny at 20C and less than 1% at 15C. Maintain by picking WT. |
| TY562 |
+/szT1 [lon-2(e678)] I; unc-32(e189) III; yDf3/szT1 X. |
C. elegans |
Unc. Throws Unc, UncLon males and dead eggs. |
| TY567 |
+/szT1 [lon-2(e678)] I; unc-32(e189) III; yDf2/szT1 X. |
C. elegans |
Unc. Throws Unc, UncLon males and dead eggs. |
| TY578 |
+/szT1 [lon-2(e678)] I; unc-32(e189) III; yDf5/szT1 X. |
C. elegans |
Unc. Throws Unc, UncLon males and dead eggs. |
| WM28 |
unc-32(e189) III; rde-1(ne219) V. |
C. elegans |
RNAi deficient. Unc. |