Variation Information: e369

Namee369 View on WormBase
Species C. elegans
Genetic positionV:24.44 +/- 0.000 cM
Genomic positionV: 20004422..20004422
Protein changeY60A3A.1 Substitution

Strains carrying this variation

Strain Genotype Species Description
AMH7 unc-51(e369) V; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. Slow growth.
AV146 chk-2(me64) rol-9(sc148)/unc-51(e369) rol-9(sc148) V. C. elegans Heterozygotes are fertile Rollers and segregate fertile non-Rollers (heterozygote), Unc Rollers (unc-51 homozygotes), and non-Unc Rollers that give 96-97% dead eggs (a high % of the survivors are males).
BE150 unc-51(e369) rol-9(sc148) V. C. elegans Roller Unc.
CB2095 dpy-1(e1) sup-5(e1877) III; unc-51(e369) V. C. elegans Dpy. Slightly Unc. Semi-dominant suppressor. Suppressed Unc between 22.5 and 24C.
CB369 unc-51(e369) V. C. elegans Paralyzed Unc. Recessive. M-MATING-NO SUCCESS.
IA260 unc-51(e369) rol-9(sc148)/sec-23(ij13) V. C. elegans Heterozygotes are WT and segregate WT, Roller Unc and embryonic lethals.
MT6025 lin-31(n301) II; dpy-9(e12) IV; unc-51(e369) V. C. elegans Mapping strain.
PJ1009 unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1256 unc-51(e369) ccIs55 V; csEx52. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. csEx52 [hsp::lin-45AA + sur-5::GFP]. Array is unstable; pick GFP+ to maintain.
PJ1257 daf-4(m592) III; unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small. Unc. Most progeny shifted to 26C form dauers.
PJ1263 gaIs37 IV; unc-51(e369) ccIs55 V. C. elegans gaIs37 [EF1a::Dmek + hs::mpk-1] IV. ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc. MuV with some frequency at 25C. Low frequency of tail defects at all temps. Appear more Dpy at 25C than 15C.
PJ1277 ccIs4251 I; unc-51(e369) ccIs55 V. C. elegans ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. ccIs55 [unc-54::lacZ + sup-7(st5)] V. GFP expression in nuclei and mitochondria of muscle cells.
PJ1283 clr-1(e1745) II; unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Clr fairly well suppressed by unc-51.
PJ1700 daf-2(m41) III; unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc -- very slow. Grows very slowly.
VC234 fre-1(gk173)/unc-51(e369) rol-9(sc148) V. C. elegans Y113G7A.8. Heterozygotes are WT and segregate WT, sterile adults (gk173 homozygotes) and UncRol progeny. Well-balanced; gk173/unc rol heterozygotes occasionally roll and are not necessarily recombinants. Pick WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2961 ttx-1(ok2889)/unc-51(e369) rol-9(sc148) V. C. elegans Y113G7A.6. Apparent homozygous lethal deletion chromosome balanced by flanking markers. Heterozygotes are WT and segregate WT, Unc-51 Rol-9 homozygotes and ok2889 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCGGGGAGTTGAATTTTG. External right primer: TTTTTCCCGAATTTTTGCAC. Internal left primer: ATGTCTTCCCGCATGAAAAT. Internal right primer: CCAGTGGTCAGAAAGCCAAT. Internal WT amplicon: 1294 bp. Deletion size: 888 bp. Deletion left flank: GTTGTTTTCTAGAAAATCTGAAAATTTTTA. Deletion right flank: TTACGAATATGAAATTTATCAAGGTCTAGG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2971 spe-19(ok3428)/unc-51(e369) rol-9(sc148) V. C. elegans Y113G7A.10. Apparent homozygous lethal deletion chromosome balanced by flanking markers. Heterozygotes are WT and segregate WT, Unc-51 Rol-9 homozygotes and ok3428 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CAAAACGTCCAAAGTGTCCC. External right primer: AGTGATTCCCAGATGTCCCA. Internal left primer: TCTCCGAAATGTCCCAGAAA. Internal right primer: CGAAAAATTCGGAAAAATCG. Internal WT amplicon: 1373 bp. Deletion size: 810 bp. Deletion left flank: ACGTCATCCTCCTGAGTTTTTTCGACTTTC. Deletion right flank: TTAAGCCTATTGAAAAGCTCTGAATTGTCC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VK1984 unc-51(e369) V; vkEx1879. C. elegans vkEx1879 [nhx-2p::cpl-1(W32A Y35A)::YFP + myo-2p::mCherry]. YFP+ accumulation in intestine. mCherry+ pharynx. Reference: Miedel MT, et al. PLoS One. 2012;7(7):e40145.