Variation Information: e1370

Namee1370 View on WormBase
Species C. elegans
Genetic positionIII:-8.13 +/- 0.000 cM
Genomic positionIII: 3000442..3000442
Protein change Substitution

Strains carrying this variation

Strain Genotype Species Description
AMH30 juIs76 II; daf-2(e1370) III. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive.
AMH36 juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C.
AMH38 juIs76 II; daf-2(e1370) III; unc-33(e204) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH46 juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. C. elegans Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow.
AMH55 daf-2(e1370) III; otIs117 IV. C. elegans otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP.
AMH67 unc-33(e204) IV; daf-2(e1370) III. C. elegans Maintain at 15C. Synthetic Lethality at 25C.
AMH69 unc-33(mn407) IV; daf-2(e1370) III. C. elegans Maintain at 15C. Synthetic Lethality at 25C.
AMH71 unc-33(e1193) IV; daf-2(e1370) III. C. elegans Maintain at 15C. Synthetic Lethality at 25C.
AUM2023 daf-2(e1370) unc-119(ed3) III; vizIs23. C. elegans vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40.
CB1370 daf-2(e1370) III. C. elegans Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY.
CF1137 daf-16(mu86) I; daf-2(e1370) III; muIs61. C. elegans muIs61 [daf-16a::GFP + rol-6(su1006)]. Temperature-sensitive. Maintain at 15 C. Rollers. Reference: Lin K, Hsin H, Libina N, Kenyon C. Nat Genet. 2001 Jun;28(2):139-45.
CF1295 daf-16(mu86) I; daf-2(e1370) III; muEx108. C. elegans muEx108 [(pKL99-2) daf-16::GFP/daf16bKO + rol-6(su1006)]. Grows okay at 20C. Rollers should form dauers at 25C. Pick Rollers to maintain.
CF1380 daf-16(mu86) I; daf-2(e1370) III; muEx158. C. elegans muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which completely rescues dauer formation and partially restores longevity of daf-16; daf-2 double mutants. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45.
CF1442 daf-16(mu86) I; daf-2(e1370) III; muEx169. C. elegans muEx169 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Pick Rollers to maintain. May grow better at 15C.
CF1449 daf-16(mu86) I; daf-2(e1370) III; muEx176. C. elegans muEx176 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Pick rollers to maintain -- Low transmission rate! Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45.
CF1514 daf-16(mu86) I; daf-2(e1370) III; muEx211. C. elegans muEx211[pNL213(ges-1p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain.
CF1515 daf-16(mu86) I; daf-2(e1370) III; muEx212. C. elegans muEx212[pNL212(myo-3p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain.
CF1580 daf-2(e1370) III; muIs84. C. elegans muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Daf-C at 25C. Can grow at 20C.
CF1588 daf-16(mu86) I; daf-2(e1370) III; muIs84. C. elegans muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Dim green expression in head, tail and around vulva. Daf-d. Can grow at 20C.
CF1595 daf-16(mu86) I; daf-2(e1370) III; muEx227. C. elegans muEx227 [(pNL213) ges-1p::GFP::daf-16) + rol-6(su1006)]. Pick Rollers to maintain.
CF1660 daf-16(mu86) I; daf-2(e1370) III; muIs84; muEx211. C. elegans muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. muEx211 [ges-1p::daf-16::GFP + rol-6(su1006)]. Pick Rollers to maintain. Partial rescue of lifespan phenotype. Some animals show variable daf-16 expression in the intestine. Grows okay at 15C. [NOTE: muEx211 is quite unstable. Be sure to pick Rollers to avoid losing the array.]
CF1724 daf-16(mu86) I; daf-2(e1370) III; muIs105. C. elegans muIs105 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Rollers; Rol phenotype is not always evident). Integrated line derived from CF1449. Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45.
CF1814 rrf-3(pk1426) II; daf-2(e1370) III. C. elegans Daf-c at 25.5C; grow at 20C or less. Long lived.
CF1827 daf-16(mu86) I; daf-2(e1370) III; muEx268. C. elegans muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. daf-16 GFP expressed in intestine. Partial rescue of lifespan phenotype. Grows okay at 15C. Pick RFP to maintain.
CF2005 daf-16(mu86) I; daf-2(e1370) III; muIs120. C. elegans muIs120 [ges-1p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Long-lived. Gamma irradiation-induced integration of muEx211. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CF2093 daf-16(mu86) I; daf-2(e1370) III; muIs131. C. elegans muIs131 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Modestly long-lived. Gamma irradiation-induced integration of muEx184. Rescues daf-16a1/c in neurons (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CF2102 daf-16(mu86) I; daf-2(e1370) III; muIs126. C. elegans muIs126 [myo-3p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Gamma irradiation-induced integration of muEx215. Rescues daf-16a1/c in muscles (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CF2570 daf-16(mu86) I; daf-2(e1370) III; muIs142. C. elegans muIs142 [ges-1p::GFP::daf-16(cDNA) + odr-1p::RFP]. Maintain at 15-20C. Gamma irradiation-induced integration of muEx268. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100.
CS624 daf-2(e1370) III; lon-2(e678) X. C. elegans Temperature-sensitive. Maintain at 15C. Lon. Daf-c.
DG2884 daf-2(e1370) III; fog-2(oz40) V. C. elegans Male-female strain. Maintain by mating at 15 C.
DR128 dpy-1(e1) daf-2(e1370) III. C. elegans Temperature sensitive dauer constitutive. Dpy. 100% dauers at 25C.
DR129 daf-2(e1370) unc-32(e189) III. C. elegans Temperature senstive dauer constitutive. Unc-coiler. 100% dauers at 25C.
DR130 dpy-1(e1) daf-2(e1370) unc-32(e189) III. C. elegans Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-2 complementation.
DR1309 daf-16(m26) I; daf-2(e1370) III. C. elegans
DR200 daf-2(e1370) III; daf-13(m66) X. C. elegans Temperature sensitive dauer constitutive. Dauers SDS-sensitive.
DR629 unc-15(e73) I; sus-1(m156) daf-2(e1370) III; eDp23 V. C. elegans Temperature sensitive dauers. Paralyzed Unc. sus-1 suppresses the ability of eDp23 to suppress unc-15. eDp23 = sup-3(e1407).
DV2275 daf-2(e1370) dpy-17(e164) III. C. elegans
ENL21 daf-2(e1370) mir-80(nDf53) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. C. elegans Maintain at 15C. Temperature-sensitive dauer constitutive (daf-c).
ENL54 daf-2(e1370) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. C. elegans Maintain at 15C. Temperature-sensitive dauer constitutive.
ENL55 daf-2(e1370) mir-80(nDf53) III; mir-81&mir-82(nDf54) X. C. elegans Maintain at 15C. Temperature-sensitive dauer constitutive.
ENL70 daf-2(e1370) III; sma-10(ok2224) IV. C. elegans Derived from RB1739 and CB1370.
GA2002 daf-2(e1370) III; wuIs305. C. elegans wuIs305 [myo-3p::Queen-2m]. Temperature sensitive dauer constitutive. Maintain at 15C. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741.
GA2003 daf-16(mgDf50) I; daf-2(e1370) III; wuIs305. C. elegans wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741.
GR1308 daf-16(mg54) I; daf-2(e1370) III. C. elegans mg54 almost completely suppresses the daf-c phenotype of daf-2. 0.4% dauers.
GR1309 daf-16(mgDf47) I; daf-2(e1370) III. C. elegans mgDf47 completely suppresses daf-c phenotype of daf-2. mgDf47 deletes approximately 8kb of the daf-16 gene beginning after exon 4.
GR1336 daf-2(e1370) III; njEx32. C. elegans njEx32 [ges-1p::daf-2(+) + ges-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50.
GR1337 daf-2(e1370) III; njEx38. C. elegans njEx38 [unc-54p::daf-2(cDNA)::unc-54 3'UTR + unc-54p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50.
GR1339 daf-2(e1370) III; mgEx376. C. elegans mgEx376 [unc-14p::daf-2 + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50.
GR1340 daf-2(e1370) III; mgEx373. C. elegans mgEx373 [unc-119p::daf-2(cDNA)::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50.
GR1895 daf-2(e1370) III; mgIs67. C. elegans mgIs67 [daf-16p::daf-16::GFP + rol-6(su1006)]. Temperature-sensitive. Daf-c. Maintain at 15C. Dauer formation at 25C. Slow growing. Dauer-like at 20C. DAF-16::GFP is fully nuclear at 20C. Reference: Riedel CG, et al. Nat Cell Biol. 2013 May;15(5):491-501.