| AMH30 |
juIs76 II; daf-2(e1370) III. |
C. elegans |
juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. |
| AMH36 |
juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. |
C. elegans |
juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C. |
| AMH38 |
juIs76 II; daf-2(e1370) III; unc-33(e204) IV. |
C. elegans |
juIs76 [unc-25p::GFP + lin-15(+)] II. |
| AMH46 |
juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. |
C. elegans |
Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow. |
| AMH55 |
daf-2(e1370) III; otIs117 IV. |
C. elegans |
otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP. |
| AMH67 |
unc-33(e204) IV; daf-2(e1370) III. |
C. elegans |
Maintain at 15C. Synthetic Lethality at 25C. |
| AMH69 |
unc-33(mn407) IV; daf-2(e1370) III. |
C. elegans |
Maintain at 15C. Synthetic Lethality at 25C. |
| AMH71 |
unc-33(e1193) IV; daf-2(e1370) III. |
C. elegans |
Maintain at 15C. Synthetic Lethality at 25C. |
| AUM2023 |
daf-2(e1370) unc-119(ed3) III; vizIs23. |
C. elegans |
vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40. |
| CB1370 |
daf-2(e1370) III. |
C. elegans |
Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY. |
| CF1137 |
daf-16(mu86) I; daf-2(e1370) III; muIs61. |
C. elegans |
muIs61 [daf-16a::GFP + rol-6(su1006)]. Temperature-sensitive. Maintain at 15 C. Rollers. Reference: Lin K, Hsin H, Libina N, Kenyon C. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1295 |
daf-16(mu86) I; daf-2(e1370) III; muEx108. |
C. elegans |
muEx108 [(pKL99-2) daf-16::GFP/daf16bKO + rol-6(su1006)]. Grows okay at 20C. Rollers should form dauers at 25C. Pick Rollers to maintain. |
| CF1380 |
daf-16(mu86) I; daf-2(e1370) III; muEx158. |
C. elegans |
muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which completely rescues dauer formation and partially restores longevity of daf-16; daf-2 double mutants. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1442 |
daf-16(mu86) I; daf-2(e1370) III; muEx169. |
C. elegans |
muEx169 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Pick Rollers to maintain. May grow better at 15C. |
| CF1449 |
daf-16(mu86) I; daf-2(e1370) III; muEx176. |
C. elegans |
muEx176 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Pick rollers to maintain -- Low transmission rate! Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1514 |
daf-16(mu86) I; daf-2(e1370) III; muEx211. |
C. elegans |
muEx211[pNL213(ges-1p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. |
| CF1515 |
daf-16(mu86) I; daf-2(e1370) III; muEx212. |
C. elegans |
muEx212[pNL212(myo-3p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. |
| CF1580 |
daf-2(e1370) III; muIs84. |
C. elegans |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Daf-C at 25C. Grows well at 20C. |
| CF1588 |
daf-16(mu86) I; daf-2(e1370) III; muIs84. |
C. elegans |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Dim green expression in head, tail and around vulva. Daf-d. Can grow at 20C. |
| CF1595 |
daf-16(mu86) I; daf-2(e1370) III; muEx227. |
C. elegans |
muEx227 [(pNL213) ges-1p::GFP::daf-16) + rol-6(su1006)]. Pick Rollers to maintain. |
| CF1660 |
daf-16(mu86) I; daf-2(e1370) III; muIs84; muEx211. |
C. elegans |
muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. muEx211 [ges-1p::daf-16::GFP + rol-6(su1006)]. Pick Rollers to maintain. Partial rescue of lifespan phenotype. Some animals show variable daf-16 expression in the intestine. Grows okay at 15C. [NOTE: muEx211 is quite unstable. Be sure to pick Rollers to avoid losing the array.] |
| CF1724 |
daf-16(mu86) I; daf-2(e1370) III; muIs105. |
C. elegans |
muIs105 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Rollers; Rol phenotype is not always evident). Integrated line derived from CF1449. Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. |
| CF1814 |
rrf-3(pk1426) II; daf-2(e1370) III. |
C. elegans |
Daf-c at 25.5C; grow at 20C or less. Long lived. |
| CF1827 |
daf-16(mu86) I; daf-2(e1370) III; muEx268. |
C. elegans |
muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. daf-16 GFP expressed in intestine. Partial rescue of lifespan phenotype. Grows okay at 15C. Pick RFP to maintain. |
| CF2005 |
daf-16(mu86) I; daf-2(e1370) III; muIs120. |
C. elegans |
muIs120 [ges-1p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Long-lived. Gamma irradiation-induced integration of muEx211. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2093 |
daf-16(mu86) I; daf-2(e1370) III; muIs131. |
C. elegans |
muIs131 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Modestly long-lived. Gamma irradiation-induced integration of muEx184. Rescues daf-16a1/c in neurons (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2102 |
daf-16(mu86) I; daf-2(e1370) III; muIs126. |
C. elegans |
muIs126 [myo-3p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Gamma irradiation-induced integration of muEx215. Rescues daf-16a1/c in muscles (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CF2570 |
daf-16(mu86) I; daf-2(e1370) III; muIs142. |
C. elegans |
muIs142 [ges-1p::GFP::daf-16(cDNA) + odr-1p::RFP]. Maintain at 15-20C. Gamma irradiation-induced integration of muEx268. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. |
| CS624 |
daf-2(e1370) III; lon-2(e678) X. |
C. elegans |
Temperature-sensitive. Maintain at 15C. Lon. Daf-c. |
| DG2884 |
daf-2(e1370) III; fog-2(oz40) V. |
C. elegans |
Male-female strain. Maintain by mating at 15 C. |
| DR128 |
dpy-1(e1) daf-2(e1370) III. |
C. elegans |
Temperature sensitive dauer constitutive. Dpy. 100% dauers at 25C. |
| DR129 |
daf-2(e1370) unc-32(e189) III. |
C. elegans |
Temperature senstive dauer constitutive. Unc-coiler. 100% dauers at 25C. |
| DR130 |
dpy-1(e1) daf-2(e1370) unc-32(e189) III. |
C. elegans |
Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-2 complementation. |
| DR1309 |
daf-16(m26) I; daf-2(e1370) III. |
C. elegans |
|
| DR200 |
daf-2(e1370) III; daf-13(m66) X. |
C. elegans |
Temperature sensitive dauer constitutive. Dauers SDS-sensitive. |
| DR629 |
unc-15(e73) I; sus-1(m156) daf-2(e1370) III; eDp23 V. |
C. elegans |
Temperature sensitive dauers. Paralyzed Unc. sus-1 suppresses the ability of eDp23 to suppress unc-15. eDp23 = sup-3(e1407). |
| DV2275 |
daf-2(e1370) dpy-17(e164) III. |
C. elegans |
|
| ENL21 |
daf-2(e1370) mir-80(nDf53) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. |
C. elegans |
Maintain at 15C. Temperature-sensitive dauer constitutive (daf-c). |
| ENL54 |
daf-2(e1370) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. |
C. elegans |
Maintain at 15C. Temperature-sensitive dauer constitutive. |
| ENL55 |
daf-2(e1370) mir-80(nDf53) III; mir-81&mir-82(nDf54) X. |
C. elegans |
Maintain at 15C. Temperature-sensitive dauer constitutive. |
| ENL70 |
daf-2(e1370) III; sma-10(ok2224) IV. |
C. elegans |
Derived from RB1739 and CB1370. |
| GA2002 |
daf-2(e1370) III; wuIs305. |
C. elegans |
wuIs305 [myo-3p::Queen-2m]. Temperature sensitive dauer constitutive. Maintain at 15C. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GA2003 |
daf-16(mgDf50) I; daf-2(e1370) III; wuIs305. |
C. elegans |
wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. |
| GR1308 |
daf-16(mg54) I; daf-2(e1370) III. |
C. elegans |
mg54 almost completely suppresses the daf-c phenotype of daf-2. 0.4% dauers. |
| GR1309 |
daf-16(mgDf47) I; daf-2(e1370) III. |
C. elegans |
mgDf47 completely suppresses daf-c phenotype of daf-2. mgDf47 deletes approximately 8kb of the daf-16 gene beginning after exon 4. |
| GR1336 |
daf-2(e1370) III; njEx32. |
C. elegans |
njEx32 [ges-1p::daf-2(+) + ges-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1337 |
daf-2(e1370) III; njEx38. |
C. elegans |
njEx38 [unc-54p::daf-2(cDNA)::unc-54 3'UTR + unc-54p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1339 |
daf-2(e1370) III; mgEx376. |
C. elegans |
mgEx376 [unc-14p::daf-2 + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1340 |
daf-2(e1370) III; mgEx373. |
C. elegans |
mgEx373 [unc-119p::daf-2(cDNA)::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. |
| GR1895 |
daf-2(e1370) III; mgIs67. |
C. elegans |
mgIs67 [daf-16p::daf-16::GFP + rol-6(su1006)]. Temperature-sensitive. Daf-c. Maintain at 15C. Dauer formation at 25C. Slow growing. Dauer-like at 20C. DAF-16::GFP is fully nuclear at 20C. Reference: Riedel CG, et al. Nat Cell Biol. 2013 May;15(5):491-501. |