| AGD1032 |
glp-1(e2141) III; xzEx1. |
C. elegans |
xzEx1 [unc-54p::Dendra2]. Maintain at 15C; sterile at 25C. Pick animals with green fluorescence in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. |
| AGD1033 |
glp-1(e2141) III; xzEx3. |
C. elegans |
xzEx3 [unc-54p::UbG76V::Dendra2]. Maintain at 15C; sterile at 25C. Pick animals with green fluorescence in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. |
| AGD1047 |
glp-1(e2141) III; uthEx649. |
C. elegans |
uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. |
| AGD1048 |
daf-16(mu86) I; glp-1(e2141) III; uthEx649. |
C. elegans |
uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. |
| AU147 |
daf-16(mgDf47) I; glp-1(e2141) III. |
C. elegans |
Temperature sensitive sterility. Maintain at 15C. |
| CB4037 |
glp-1(e2141) III. |
C. elegans |
Temperature sensitive. Sterile at 25C. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. [2/98: Craig Mello noticed a different embryonic phenotype in this strain as compared to the e2141 stock that Jim Priess obtained from England-the ABp fate appears WT.] [NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2144) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in Worm Breeders Gazette December 2010; 18(3), e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A.] |
| CF1880 |
daf-16(mu86) I; glp-1(e2141) III. |
C. elegans |
Sterile at 25C; grow at 20C or less. glp-1(e2141) longevity suppressed by daf-16(mu86). |
| CF1935 |
daf-16(mu86) I; glp-1(e2141) III; muIs109. |
C. elegans |
muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. |
| CF2061 |
daf-16(mu86) I; glp-1(e2141) III; muEx158. |
C. elegans |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2065 |
kri-1(ok1251) I; glp-1(e2141) III. |
C. elegans |
Temperature-sensitive. Sterile at 25C. [NOTE: can be grown at 20C, but 15C might be better for long-term propagation.] kri-1(ok1251) suppresses the longevity of glp-1(e2141) mutants. |
| CF2135 |
daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muIs109. |
C. elegans |
muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. |
| CF2154 |
tcer-1(tm1452) II; glp-1(e2141) III. |
C. elegans |
Temperature sensitive sterile at 25C. tcer-1(-) causes loss of germ cells and increases lifespan. Reference: Ghazi A, Henis-Korenbilt S, & Kenyon C (2009) PLoS Genet 5(9):e1000639. |
| CF2247 |
daf-16(mu86) I; glp-1(e2141) III; muEx248. |
C. elegans |
muEx248 [daf-16::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| CF2248 |
daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx158. |
C. elegans |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2263 |
daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx158. |
C. elegans |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2265 |
daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muEx158. |
C. elegans |
muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. |
| CF2278 |
daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. |
C. elegans |
muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| CF2288 |
daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx248. |
C. elegans |
muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. |
| CF2310 |
kri-1(ok1251) I; glp-1(e2141) III; muEx353. |
C. elegans |
muEx353 [kri-1(cDNA, a-isoform)::GFP + odr-1p::RFP]. Pick RFP+ to maintain. Temperature sensitive. Sterile at 25C; maintain at 15-20C. Reference: Berman JR and Kenyon C. Cell. 2006 Mar 10;124(5):1055-68. |
| EU367 |
glp-1(e2141) III; mom-2(or42) IV/nT1 [unc-?(n754) let-?] (IV;V). |
C. elegans |
Maintain at 15C. Animals are Unc. |
| HGA8002 |
glp-1(e2141) III; lynEx1. |
C. elegans |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1 alone. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8004 |
daf-16(mu86) I; glp-1(e2141) III; lynEx1. |
C. elegans |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of daf-16; glp-1 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8005 |
glp-1(e2141) III; daf-12(rh61rh411) X; lynEx1. |
C. elegans |
lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1; daf-12 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8006 |
glp-1(e2141) III; fat-5 (tm420) V. |
C. elegans |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8007 |
glp-1(e2141) III; fat-6 (tm331) IV. |
C. elegans |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8008 |
glp-1(e2141) III; fat-7 (wa36) V. |
C. elegans |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8009 |
glp-1(e2141) III; fat-5 (tm420) fat-7 (wa36) V. |
C. elegans |
Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| HGA8012 |
glp-1(e2141) III; daf-9(rh50) X; lynEx1. |
C. elegans |
lynEx1 [nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 15-20C. Temperature-sensitve Daf-c. Mig. Pick DsRed+ animals to maintain. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. |
| MAH50 |
daf-16(mu86) I; glp-1(e2141) III; adIs2122. |
C. elegans |
adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Maintain at 15C or 20C. Sterile at 25C. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH88 |
glp-1(e2141) III; mgEx779. |
C. elegans |
mgEx779 [lipl-4p::lipl-4::SL2::GFP + myo-2p::mCherry]. Pick RFP+ to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| SD1966 |
glp-1(e2141) III; gaIs290. |
C. elegans |
gaIs290 [elt-2::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Temperature-sensitive sterile. Maintain at 15C. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Recombineered fosmid was integrated by biolistic bombardment. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Mann F, et al. PLoS. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org) |
