Variation Information: e2141

Namee2141 View on WormBase
Species C. elegans
Genetic positionIII:0.16 +/- 0.000 cM
Genomic positionIII: 9098313..9098313
Protein changeF02A9.6 Substitution

Strains carrying this variation

Strain Genotype Species Description
AGD1032 glp-1(e2141) III; xzEx1. C. elegans xzEx1 [unc-54p::Dendra2]. Maintain at 15C; sterile at 25C. Pick animals with green fluorescence in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8.
AGD1033 glp-1(e2141) III; xzEx3. C. elegans xzEx3 [unc-54p::UbG76V::Dendra2]. Maintain at 15C; sterile at 25C. Pick animals with green fluorescence in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8.
AGD1047 glp-1(e2141) III; uthEx649. C. elegans uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8.
AGD1048 daf-16(mu86) I; glp-1(e2141) III; uthEx649. C. elegans uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8.
AU147 daf-16(mgDf47) I; glp-1(e2141) III. C. elegans Temperature sensitive sterility. Maintain at 15C.
CB4037 glp-1(e2141) III. C. elegans Temperature sensitive. Sterile at 25C. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. [2/98: Craig Mello noticed a different embryonic phenotype in this strain as compared to the e2141 stock that Jim Priess obtained from England-the ABp fate appears WT.] [NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2144) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in Worm Breeders Gazette December 2010; 18(3), e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A.]
CF1880 daf-16(mu86) I; glp-1(e2141) III. C. elegans Sterile at 25C; grow at 20C or less. glp-1(e2141) longevity suppressed by daf-16(mu86).
CF1935 daf-16(mu86) I; glp-1(e2141) III; muIs109. C. elegans muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less.
CF2061 daf-16(mu86) I; glp-1(e2141) III; muEx158. C. elegans muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less.
CF2065 kri-1(ok1251) I; glp-1(e2141) III. C. elegans kr-1(ok1251) suppresses the longevity of glp-1(e2141) mutants. Sterile at 25C; grow at 20C or less.
CF2135 daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muIs109. C. elegans muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less.
CF2154 tcer-1(tm1452) II; glp-1(e2141) III. C. elegans Temperature sensitive sterile at 25C. tcer-1(-) causes loss of germ cells and increases lifespan. Reference: Ghazi A, Henis-Korenbilt S, & Kenyon C (2009) PLoS Genet 5(9):e1000639.
CF2247 daf-16(mu86) I; glp-1(e2141) III; muEx248. C. elegans muEx248 [daf-16::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.
CF2248 daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx158. C. elegans muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less.
CF2263 daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx158. C. elegans muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less.
CF2265 daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muEx158. C. elegans muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less.
CF2278 daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. C. elegans muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.
CF2288 daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx248. C. elegans muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.
CF2310 kri-1(ok1251) I; glp-1(e2141) III; muEx353. C. elegans muEx353 [kri-1(cDNA, a-isoform)::GFP + odr-1p::RFP]. Pick RFP+ to maintain. Temperature sensitive. Sterile at 25C; maintain at 15-20C. Reference: Berman JR and Kenyon C. Cell. 2006 Mar 10;124(5):1055-68.
EU367 glp-1(e2141) III; mom-2(or42) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Maintain at 15C. Animals are Unc.
HGA8002 glp-1(e2141) III; lynEx1. C. elegans lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1 alone. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8004 daf-16(mu86) I; glp-1(e2141) III; lynEx1. C. elegans lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of daf-16; glp-1 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8005 glp-1(e2141) III; daf-12(rh61rh411) X; lynEx1. C. elegans lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1; daf-12 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8006 glp-1(e2141) III; fat-5 (tm420) V. C. elegans Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8007 glp-1(e2141) III; fat-6 (tm331) IV. C. elegans Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8008 glp-1(e2141) III; fat-7 (wa36) V. C. elegans Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8009 glp-1(e2141) III; fat-5 (tm420) fat-7 (wa36) V. C. elegans Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
HGA8012 glp-1(e2141) III; daf-9(rh50) X; lynEx1. C. elegans lynEx1 [nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 15-20C. Temperature-sensitve Daf-c. Mig. Pick DsRed+ animals to maintain. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599.
MAH50 daf-16(mu86) I; glp-1(e2141) III; adIs2122. C. elegans adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Maintain at 15C or 20C. Sterile at 25C. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14.
MAH88 glp-1(e2141) III; mgEx779. C. elegans mgEx779 [lipl-4p::lipl-4::SL2::GFP + myo-2p::mCherry]. Pick RFP+ to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14.
SD1966 glp-1(e2141) III; gaIs290. C. elegans gaIs290 [elt-2::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Temperature-sensitive sterile. Maintain at 15C. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Recombineered fosmid was integrated by biolistic bombardment. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Mann F, et al. PLoS. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)