PT559 |
nphp-1(ok500) II; him-5(e1490) V. |
C. elegans |
Superficially WT. |
PT709 |
nphp-4(tm925) him-5(e1490) V. |
C. elegans |
Superficially WT. |
PT8 |
pkd-2(sy606) IV; him-5(e1490) V. |
C. elegans |
Males are response and location-of-vulva defective. pkd-2(sy606) is a 2396 bp deletion (8388 to 5942 in YAC Y73F8A); mutant protein is predicted to contain the N-terminal portion of the protein midway through the first predicted transmembrane region. |
PT830 |
nphp-1(ok500) II; nphp-4(tm925) him-5(e1490) V. |
C. elegans |
Male mating response defect. |
PX623 |
fxDf1 II; him-5(e1490) V. |
C. elegans |
fxDf1 (II: 2,484,339 - 2,487,244) removes nspf-1, nspf-2, and nspf-3. Him. This strain carries a knockout of the Nematode-Specific Peptide family, group F (NSPF) gene family, which localizes to sperm membranous organelles. There are no effects on spermatogenesis, male fertility, or sperm competitive ability. Hermaphrodites produce approximately 30% males. Reference: Kasimatis KR, et al. (2018) BioRxiv 290221; doi: https://doi.org/10.1101/290221. |
PX629 |
fxIs1 I; spe-44(fx110[spe-44::degron]) IV; him-5(e1490) V. |
C. elegans |
fxIs1 [pie-1p::TIR1::mRuby, I:2851009] I. Auxin-inducible spermatogenesis arrest, resulting in hermaphrodite self-sterility and reversible male sterility. Him: males produced at ~30%. Degron tag was inserted into the endogenous spe-44 locus. Reference: Kasimatis KR, et al. (2018) Auxin-Mediated Sterility Induction System for Longevity and Mating Studies in Caenorhabditis elegans. BioRvix. doi: https://doi.org/10.1101/284232. |
RA334 |
unc-119(ed3) III; him-5(e1490) V; rdIs26. |
C. elegans |
rdIs26 [R08E3.4::GFP + unc-119(+)]. Construct contains ~5 kb upstream of R08E3.4A. Superficially wild-type. Reference: Large and Mathies (2010) Dev Biol 339(1):51-64. |
RA335 |
unc-119(ed3) III; him-5(e1490) V; rdIs27. |
C. elegans |
rdIs27 [R08E3.4::GFP + unc-119(+)]. Construct contains ~5 kb upstream of R08E3.4A. Superficially wild-type. Reference: Large and Mathies (2010) Dev Biol 339(1):51-64. |
RJP255 |
ynIs34 IV; him-5(e1490) V. |
C. elegans |
ynIs34 [flp-19p::GFP] IV. Him. Transcriptional flp-19 reporter. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. Clark SG & Chiu C. Development. 2003 Aug;130(16):3781-94. Kim K & Li C. J Comp Neurol. 2004 Aug 2;475(4):540-50. |
SL438 |
spe-9(eb19) I; him-5(e1490) V; ebEx126. |
C. elegans |
ebEx126 [YAC Y47H9 [spe-9(+)] + rol-6(su1006)]. Pick Rollers to maintain. eb19 is a spe-9 non-conditional mutant. |
SM1584 |
mxl-2(tm1516) III; plx-1(nc37) IV; him-5(e1490) V; bar-1(ga80) X. |
C. elegans |
Hermaphrodites are sluggish and exhibit protruding vulva, ruptured vulva, or internally hatched progeny. Males move slower than WT and have disorganized tail rays. |
SM1585 |
plx-1(nc37) IV; him-5(e1490) V; bar-1(ga80) X. |
C. elegans |
Hermaphrodites are sluggish and exhibit protruding vulva, ruptured vulva, or internally hatched progeny. Males move slower than WT and have disorganized tail rays. |
SM1586 |
mxl-2(tm1516) III; plx-1(nc37) IV; him-5(e1490) V. |
C. elegans |
Hermaphrodites seem fine. Males have a high penetrance of anterior displacement of ray 1. |
SP1933 |
unc-29(e1072) I; him-5(e1490) V. |
C. elegans |
|
SS230 |
unc-13(e51) I; him-5(e1490) V; nDp4 (I;V)/+. |
C. elegans |
Animals with the Duplication are WT. Animals which have lost the Duplication are Unc. Throws males. |
ST53 |
ncIs3 III; him-5(e1490) V. |
C. elegans |
ncIs3 [pH20::GFP + pBlueScript]. Expresses GFP in nearly all neurons. No morphological or behavioral phenotypes. |
ST54 |
plx-1(nc37) IV; him-5(e1490) V. |
C. elegans |
Various epidermal defects. In male tails, ray 1 is dislocated anteriorly. |
TU3568 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. |
C. elegans |
uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
TU3595 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. |
C. elegans |
uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette. |
TU3596 |
sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. |
C. elegans |
Him. Enhanced RNAi background. Maintain under normal conditions. |
UR109 |
cwp-4(tm727) him-5(e1490) V. |
C. elegans |
Him strain. Superficially wild-type. References: Portman and Emmons Dev Bio (2004) & Miller and Portman Mod & Mech (2010). |
UR110 |
cwp-2&cwp-3(ok1366) him-5(e1490) V. |
C. elegans |
Him strain. Superficially wild-type. References: Portman and Emmons Dev Bio (2004) & Miller and Prtman Mod & Mech (2010). |
UR116 |
him-5(e1490) V; cwp-5(tm1893) X. |
C. elegans |
Him strain. Superficially wild-type. Males have mating (response) defect but are fertile; otherwise superficially wild-type. Reference: Miller and Prtman Mod & Mech (2010). |
WS841 |
ptp-2(op194) unc-4(e120)/mIn1 [dpy-10(e128)] II; him-5(e1490) V. |
C. elegans |
Heterozygotes are WT and segregate WT, Uncs which are sterile (>10 offspring) and Dpys. Throws males of all classes. mIn1 pka mC6. |