Variation Information: e1490

Namee1490 View on WormBase
Species C. elegans
Genetic positionV:6.09 +/- 0.018 cM
Genomic positiongenomic coordinates unknown or not listed
Protein changeprotein change unknown or not listed

Strains carrying this variation

Strain Genotype Species Description
HZ455 him-5(e1490) V; bpIs131. C. elegans bpIs131 [sepa-1p::sepa-1::GFP + unc-76(+)]. Him. SEPA-1::GFP aggregates form in a temporal pattern. Diffuse SEPA-1::GFP is detectible in most cells at the comma stage and greatly diminished by the 2-fold stage. After hatching, cytoplasmic SEPA-1::GFP aggregates were found in a few unidentified cells in the head and tail regions and also in the intestine, especially in the anterior and posterior pairs of intestine cells. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55.
HZ504 him-5(e1490) V; bpIs37. C. elegans bpIs37 [dcap-1p::dcap-1::DsRed + rol-6(su1006)]. Rollers. Translational DsRed reporter for the P body-specific marker dcap-1, which encodes the C. elegans ortholog of decapping complex component DCAP1. Low levels of expression are homogenously distributed in the cytoplasm at all stages of embryogenesis. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ589 him-5(e1490) V; bpIs151. C. elegans bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. Him. In wild-type embryos, sqst-1::GFP is very weakly expressed and diffusely localized in the cytoplasm. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55.
HZ66 sop-2(bp186) II; him-5(e1490) bxIs16 V. C. elegans bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ67 him-5(e1490) bxIs16 V; sor-3(bp185) X. C. elegans bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ769 him-5(e1490) V; bpIs88. C. elegans bpIs88 [tia-1p::tia-1::GFP + dcap-1p::dcap-1::RFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ771 him-5(e1490) V; bpIs90. C. elegans bpIs90 [tia-1p::tia-1::GFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. TIA-1::GFP is homogenously distributed in the cytoplasm during embryogenesis. At larval stages, diffuse TIA-1::GFP signals are observed in seam cells and hypodermal cells. TIA-1::GFP forms distinct bodies, especially in seam cells and hypodermal cells, under stress conditions. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
IC400 zdIs5 I; quIs5 II; him-5(e1490) V. C. elegans zdIs5 [mec-4::GFP + lin-15(+)]. quIs5 [mec-4::myr::vab-1 + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons.
JJ1057 pop-1(zu189) dpy-5(e61)/hT1 I; him-5(e1490)/hT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, mid-larval lethals (hT1 homozygotes), males and dead eggs. Mutation in pop-1 results in the MS blastomere adopting the fate of the E blastomere. pop-1 mutant embryos have twice the amount of wild type gut and only make anterior pharynx. him-5 is outside the region balanced by hT2.
JK2505 cyd-1(q626) II; him-5(e1490) V. C. elegans Temperature-sensitive allele of cyd-1. Phenotypically wild-type at 15C. At 25C, approximately one-third of q626 hermaphrodites were missing one distal tip cell (DTC) and approximately one-half of q626 males were missing the linker cell (LC). q626 also feminizes the XO gonad. q626 affects the production of SGP daughters in both sexes. There is also a maternal component since the mutant phenotype is almost fully penetrant in offspring of homozygous mothers, but less penetrant in offspring of heterozygous mothers. Reference: Tilmann C & Kimble J. Dev Cell. 2005 Oct;9(4):489-99.
JK3375 gld-3(q730)/mIn1 [mIs14 dpy-10(e128)] II; him-5(e1490) V. C. elegans Heterozygotes are WT with major GFP signal in pharynx. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and GFP- gld-3 homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK3437 him-5(e1490) V; qIs74. C. elegans qIs74 [sys-1p::GFP::pop-1 + unc-119(+)]. Probably on LG X. May have unc-119 in background. Throws 30% males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK590 glp-1(q35)/eT1 III; him-5(e1490)/eT1 V. C. elegans Heterozygotes are wild-type and segregate wild-type heterozygotes, glp-1(q35) homozygotes (Muv steriles), eT1 homozygotes (Unc-36), and males. glp-1(q35) has a semi-dominant multi-vulva phenotype as well as the loss-of-function Glp phenotype (sterility and embryonic lethality). The q35 mutation is a nonsense mutation that eliminates 122 C-terminal amino acids including a PEST sequence. The C terminus was thought to contain a negative regulatory domain that inactivates glp-1 in the VPCs; the inappropriate glp-1(q35) activity can substitute for lin-12 vulval fate determination. References: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mango S, et al. Nature. 1991 Aug 29;352(6338):811-5.
JT8132 lin-49(sa470) IV; him-5(e1490) V. C. elegans Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT8193 lin-59(sa489) I; him-5(e1490) V. C. elegans Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT9461 lin-48(sa469) III; him-5(e1490) V. C. elegans Male spicule defect.
KC565 sin-3(tm1276) I; him-5(e1490) V. C. elegans Mab. Pvl. Throws males.
KS411 lin-17(n671) I; unc-119(e2498) III; him-5(e1490) V; mhIs9. C. elegans mhIs9 [lin-17::GFP]. Full length lin-17, expressed T.p cells. Rescues lin-17 mutants. unc-119(e2498) may no longer be in the background.
KWN117 pha-1(e2123) III; him-5(e1490) V; rnyEx60. C. elegans rnyEx60 [vha-6p:::vha-6::mCherry + myo-3p::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Labels apical intestinal membrane. Reference: Allman et al. (2009) Am J Physiol Cell 297:C1071-81.
KWN190 pha-1(e2123) III; him-5(e1490) V; rnyEx109. C. elegans rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613.
MH1157 him-5(e1490) V; egl-13(ku194) X. C. elegans ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194).
MH1946 dli-1(ku266)/+ IV; him-5(e1490) V. C. elegans Heterozygotes are WT and segregate WT and Sterile animals with a protruding vulva. Throws males. Clone several WT to recover heterozygote. Cytoplasmic dynein light intermediate chain. 9/02: ku266 is not an L to stop; it is a W117 to a stop (TGG to TGA). Sandhya Koushika.
MT2537 dpy-13(e184) unc-8(n491) IV; him-5(e1490) V. C. elegans DpyUnc. e184 and n491 are both semi-dominant.
MT4051 lin-44(n1792) I; him-5(e1490) V. C. elegans At 20C about 50% of the hermaphrodites are Egl. Males have an abnormal tail and do not mate.
MT4150 unc-17(e245) dpy-4(e1166) IV; him-5(e1490) V. C. elegans DpyUnc. Segregates males.
MT4156 lin-26(n156)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. C. elegans Heterozygotes are WT and segregate WT, Egls with abnormal tails and DpyUncs.
MT4700 let-60(n1531) unc-22(e66)/let-60(n1700) dpy-20(e1282) IV; him-5(e1490) V. C. elegans Heterozygotes are mostly non-Muv (about 20% will be Muv because n1700 is semi-dominant) and non-Unc. Hets segregate Twitchers which die at various larval stages or as Vul adults, because of the maternal effect rescue of n1531 by n1700. The Twitchers will not give viable progeny. Hets also segregate DpyMuvs. The strain segregates males which will mate, though not very well.
MT4705 lin-17(n671) lin-44(n1792) I; him-5(e1490) V. C. elegans
MT4718 egl-31(n472) I; him-5(e1490) V. C. elegans Egg laying defective. Makes bags of worms. Backward Unc. Throws males.
MT5726 dpy-20(e1362) unc-22(e66) IV; him-5(e1490) V; nDp5 (IV;f). C. elegans Animals with the Duplication are WT. Animals which have lost the Duplication are Dpy and Twitchers. Throws both WT and DpyTwitcher males. Maintain by picking WT.
MT5823 lin-26(n156) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. C. elegans Heterozygotes are WT.
NG2517 him-5(e1490) V; gmIs5. C. elegans gmIs5 [ina-1::GFP + rol-6(su1006)]. Rollers. ina-1(gm86) may still be in the background.
NW1550 efn-2(ev658) him-5(e1490) C. elegans Male ray fusions: approx 15% penetrant.
NW1615 plx-1(ev724) jcIs1 IV; him-5(e1490) V. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW1696 him-5(e1490) V; evIs138. C. elegans evIs138 [plx-2(+) + sur-5::GFP]. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1697 plx-2(ev775) II; him-5(e1490) V. C. elegans Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1698 plx-2(ev774) II; him-5(e1490) V. C. elegans Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1699 plx-2(ev738) II; him-5(e1490) V. C. elegans Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1700 plx-2(ev773) II; him-5(e1490) V. C. elegans Null allele. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. Some embryonic and larval lethality.
NW1701 mab-20(ev778) I; muIs32 II; him-5(e1490) V. C. elegans muIs32 [mec-7p::GFP + lin-15(+)]. Extensive ray fusion. Body morphology defects at larval stages. Embryonic and larval lethality.
NW1702 smp-1(ev715) I; jcIs1 IV; him-5(e1490) V. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-2(ev709). Vulva cell migration defects. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW1703 him-5(e1490) V; evEx193. C. elegans evEx193 [semala::GFP]. GFP fluorescence in interneurons (AVL), the excretory channel, the distal tip cells (DTC), somatic cells of the gonad, epidermal cells. In adult, expression in the fused seam cell syncitium.
NW1704 smp-2(ev709) I; jcIs1 IV; him-5(e1490) V. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-1(ev715). ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NY2045 ynIs45 I; him-5(e1490) V. C. elegans ynIs45 [flp-15p::GFP].
NY2054 ynIs54 IV; him-5(e1490) V. C. elegans ynIs54 [flp-20p::GFP]. Him.
NY2057 ynIs57 III; him-5(e1490) V. C. elegans ynIs57 [flp-2p::GFP].
NY2064 ynIs64 I; him-5(e1490) V. C. elegans ynIs64 [flp-17p::GFP].
NY2066 ynIs66 IV; him-5(e1490) V. C. elegans ynIs66 [flp-7p::GFP].
NY2067 ynIs67 III; him-5(e1490) V. C. elegans ynIs67 [flp-6p::GFP].
OH10196 ceh-43(tm480) III; him-5(e1490) V; nIs118; norEx41. C. elegans nIs118 [cat-2::GFP]. norEx41 [ceh-43 fosmid + dat-1::mCherry]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain.