| HZ455 |
him-5(e1490) V; bpIs131. |
C. elegans |
bpIs131 [sepa-1p::sepa-1::GFP + unc-76(+)]. Him. SEPA-1::GFP aggregates form in a temporal pattern. Diffuse SEPA-1::GFP is detectible in most cells at the comma stage and greatly diminished by the 2-fold stage. After hatching, cytoplasmic SEPA-1::GFP aggregates were found in a few unidentified cells in the head and tail regions and also in the intestine, especially in the anterior and posterior pairs of intestine cells. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55. |
| HZ504 |
him-5(e1490) V; bpIs37. |
C. elegans |
bpIs37 [dcap-1p::dcap-1::DsRed + rol-6(su1006)]. Rollers. Translational DsRed reporter for the P body-specific marker dcap-1, which encodes the C. elegans ortholog of decapping complex component DCAP1. Low levels of expression are homogenously distributed in the cytoplasm at all stages of embryogenesis. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39. |
| HZ589 |
him-5(e1490) V; bpIs151. |
C. elegans |
bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. Him. In wild-type embryos, sqst-1::GFP is very weakly expressed and diffusely localized in the cytoplasm. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55. |
| HZ66 |
sop-2(bp186) II; him-5(e1490) bxIs16 V. |
C. elegans |
bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C. |
| HZ67 |
him-5(e1490) bxIs16 V; sor-3(bp185) X. |
C. elegans |
bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C. |
| HZ769 |
him-5(e1490) V; bpIs88. |
C. elegans |
bpIs88 [tia-1p::tia-1::GFP + dcap-1p::dcap-1::RFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39. |
| HZ771 |
him-5(e1490) V; bpIs90. |
C. elegans |
bpIs90 [tia-1p::tia-1::GFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. TIA-1::GFP is homogenously distributed in the cytoplasm during embryogenesis. At larval stages, diffuse TIA-1::GFP signals are observed in seam cells and hypodermal cells. TIA-1::GFP forms distinct bodies, especially in seam cells and hypodermal cells, under stress conditions. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39. |
| IC400 |
zdIs5 I; quIs5 II; him-5(e1490) V. |
C. elegans |
zdIs5 [mec-4::GFP + lin-15(+)]. quIs5 [mec-4::myr::vab-1 + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons. |
| JJ1057 |
pop-1(zu189) dpy-5(e61)/hT1 I; him-5(e1490)/hT1 V. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, mid-larval lethals (hT1 homozygotes), males and dead eggs. Mutation in pop-1 results in the MS blastomere adopting the fate of the E blastomere. pop-1 mutant embryos have twice the amount of wild type gut and only make anterior pharynx. him-5 is outside the region balanced by hT2. |
| JK2505 |
cyd-1(q626) II; him-5(e1490) V. |
C. elegans |
Temperature-sensitive allele of cyd-1. Phenotypically wild-type at 15C. At 25C, approximately one-third of q626 hermaphrodites were missing one distal tip cell (DTC) and approximately one-half of q626 males were missing the linker cell (LC). q626 also feminizes the XO gonad. q626 affects the production of SGP daughters in both sexes. There is also a maternal component since the mutant phenotype is almost fully penetrant in offspring of homozygous mothers, but less penetrant in offspring of heterozygous mothers. Reference: Tilmann C & Kimble J. Dev Cell. 2005 Oct;9(4):489-99. |
| JK3375 |
gld-3(q730)/mIn1 [mIs14 dpy-10(e128)] II; him-5(e1490) V. |
C. elegans |
Heterozygotes are WT with major GFP signal in pharynx. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and GFP- gld-3 homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK3437 |
him-5(e1490) V; qIs74. |
C. elegans |
qIs74 [sys-1p::GFP::pop-1 + unc-119(+)]. Probably on LG X. May have unc-119 in background. Throws 30% males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| JK590 |
glp-1(q35)/eT1 III; him-5(e1490)/eT1 V. |
C. elegans |
Heterozygotes are wild-type and segregate wild-type heterozygotes, glp-1(q35) homozygotes (Muv steriles), eT1 homozygotes (Unc-36), and males. glp-1(q35) has a semi-dominant multi-vulva phenotype as well as the loss-of-function Glp phenotype (sterility and embryonic lethality). The q35 mutation is a nonsense mutation that eliminates 122 C-terminal amino acids including a PEST sequence. The C terminus was thought to contain a negative regulatory domain that inactivates glp-1 in the VPCs; the inappropriate glp-1(q35) activity can substitute for lin-12 vulval fate determination. References: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mango S, et al. Nature. 1991 Aug 29;352(6338):811-5. |
| JT8132 |
lin-49(sa470) IV; him-5(e1490) V. |
C. elegans |
Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938. |
| JT8193 |
lin-59(sa489) I; him-5(e1490) V. |
C. elegans |
Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938. |
| JT9461 |
lin-48(sa469) III; him-5(e1490) V. |
C. elegans |
Male spicule defect. |
| KC565 |
sin-3(tm1276) I; him-5(e1490) V. |
C. elegans |
Mab. Pvl. Throws males. |
| KS411 |
lin-17(n671) I; unc-119(e2498) III; him-5(e1490) V; mhIs9. |
C. elegans |
mhIs9 [lin-17::GFP]. Full length lin-17, expressed T.p cells. Rescues lin-17 mutants. unc-119(e2498) may no longer be in the background. |
| KWN117 |
pha-1(e2123) III; him-5(e1490) V; rnyEx60. |
C. elegans |
rnyEx60 [vha-6p:::vha-6::mCherry + myo-3p::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Labels apical intestinal membrane. Reference: Allman et al. (2009) Am J Physiol Cell 297:C1071-81. |
| KWN190 |
pha-1(e2123) III; him-5(e1490) V; rnyEx109. |
C. elegans |
rnyEx109 [nhx-2p::D3cpv + pha-1(+)]. Maintain at 20-25C to select for array. KWN190 strain expresses the FRET-based calcium indicator protein D3cpv throughout the cytoplasm of intestinal cells. Dual emission ratio imaging (ex. 435, em. 480/535-nm) can be used to measure intestinal calcium and, although the FRET pair is CFP/YFP, intestinal D3cpv fluorescence is observable under standard GFP filter sets. The D3cpv biosensor has the advantages of being relatively pH-insensitive and not interfering with endogenous calmodulin signaling. References: Am J Physiol Cell Physiol. 301:C1389-1403. Am J Physiol Cell Physiol. 297:C1071-81. FASEB J. 27:760-768. PLoS Biol. 11: e1001613. |
| MH1157 |
him-5(e1490) V; egl-13(ku194) X. |
C. elegans |
ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194). |
| MH1946 |
dli-1(ku266)/+ IV; him-5(e1490) V. |
C. elegans |
Heterozygotes are WT and segregate WT and Sterile animals with a protruding vulva. Throws males. Clone several WT to recover heterozygote. Cytoplasmic dynein light intermediate chain. 9/02: ku266 is not an L to stop; it is a W117 to a stop (TGG to TGA). Sandhya Koushika. |
| MT2537 |
dpy-13(e184) unc-8(n491) IV; him-5(e1490) V. |
C. elegans |
DpyUnc. e184 and n491 are both semi-dominant. |
| MT4051 |
lin-44(n1792) I; him-5(e1490) V. |
C. elegans |
At 20C about 50% of the hermaphrodites are Egl. Males have an abnormal tail and do not mate. |
| MT4150 |
unc-17(e245) dpy-4(e1166) IV; him-5(e1490) V. |
C. elegans |
DpyUnc. Segregates males. |
| MT4156 |
lin-26(n156)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. |
C. elegans |
Heterozygotes are WT and segregate WT, Egls with abnormal tails and DpyUncs. |
| MT4700 |
let-60(n1531) unc-22(e66)/let-60(n1700) dpy-20(e1282) IV; him-5(e1490) V. |
C. elegans |
Heterozygotes are mostly non-Muv (about 20% will be Muv because n1700 is semi-dominant) and non-Unc. Hets segregate Twitchers which die at various larval stages or as Vul adults, because of the maternal effect rescue of n1531 by n1700. The Twitchers will not give viable progeny. Hets also segregate DpyMuvs. The strain segregates males which will mate, though not very well. |
| MT4705 |
lin-17(n671) lin-44(n1792) I; him-5(e1490) V. |
C. elegans |
|
| MT4718 |
egl-31(n472) I; him-5(e1490) V. |
C. elegans |
Egg laying defective. Makes bags of worms. Backward Unc. Throws males. |
| MT5726 |
dpy-20(e1362) unc-22(e66) IV; him-5(e1490) V; nDp5 (IV;f). |
C. elegans |
Animals with the Duplication are WT. Animals which have lost the Duplication are Dpy and Twitchers. Throws both WT and DpyTwitcher males. Maintain by picking WT. |
| MT5823 |
lin-26(n156) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-5(e1490) V. |
C. elegans |
Heterozygotes are WT. |
| NG2517 |
him-5(e1490) V; gmIs5. |
C. elegans |
gmIs5 [ina-1::GFP + rol-6(su1006)]. Rollers. ina-1(gm86) may still be in the background. |
| NW1550 |
efn-2(ev658) him-5(e1490) |
C. elegans |
Male ray fusions: approx 15% penetrant. |
| NW1615 |
plx-1(ev724) jcIs1 IV; him-5(e1490) V. |
C. elegans |
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91. |
| NW1696 |
him-5(e1490) V; evIs138. |
C. elegans |
evIs138 [plx-2(+) + sur-5::GFP]. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. |
| NW1697 |
plx-2(ev775) II; him-5(e1490) V. |
C. elegans |
Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. |
| NW1698 |
plx-2(ev774) II; him-5(e1490) V. |
C. elegans |
Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. |
| NW1699 |
plx-2(ev738) II; him-5(e1490) V. |
C. elegans |
Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. |
| NW1700 |
plx-2(ev773) II; him-5(e1490) V. |
C. elegans |
Null allele. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. Some embryonic and larval lethality. |
| NW1701 |
mab-20(ev778) I; muIs32 II; him-5(e1490) V. |
C. elegans |
muIs32 [mec-7p::GFP + lin-15(+)]. Extensive ray fusion. Body morphology defects at larval stages. Embryonic and larval lethality. |
| NW1702 |
smp-1(ev715) I; jcIs1 IV; him-5(e1490) V. |
C. elegans |
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-2(ev709). Vulva cell migration defects. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91. |
| NW1703 |
him-5(e1490) V; evEx193. |
C. elegans |
evEx193 [semala::GFP]. GFP fluorescence in interneurons (AVL), the excretory channel, the distal tip cells (DTC), somatic cells of the gonad, epidermal cells. In adult, expression in the fused seam cell syncitium. |
| NW1704 |
smp-2(ev709) I; jcIs1 IV; him-5(e1490) V. |
C. elegans |
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-1(ev715). ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91. |
| NY2045 |
ynIs45 I; him-5(e1490) V. |
C. elegans |
ynIs45 [flp-15p::GFP]. |
| NY2054 |
ynIs54 IV; him-5(e1490) V. |
C. elegans |
ynIs54 [flp-20p::GFP]. Him. |
| NY2057 |
ynIs57 III; him-5(e1490) V. |
C. elegans |
ynIs57 [flp-2p::GFP]. |
| NY2064 |
ynIs64 I; him-5(e1490) V. |
C. elegans |
ynIs64 [flp-17p::GFP]. |
| NY2066 |
ynIs66 IV; him-5(e1490) V. |
C. elegans |
ynIs66 [flp-7p::GFP]. |
| NY2067 |
ynIs67 III; him-5(e1490) V. |
C. elegans |
ynIs67 [flp-6p::GFP]. |
| OH10196 |
ceh-43(tm480) III; him-5(e1490) V; nIs118; norEx41. |
C. elegans |
nIs118 [cat-2::GFP]. norEx41 [ceh-43 fosmid + dat-1::mCherry]. Him. tm480 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain. |