Transgene Information
Name | olaEx3013 View on WormBase |
---|---|
Description | [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry] |
Reporter | EGFP |
Strains carrying this transgene
Strain | Genotype | Species | Description |
---|---|---|---|
AMH57 | unc-33(mn407); olaEx3013. | C. elegans | olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 |
AMH59 | unc-33(e204); olaEx3013. | C. elegans | olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Paralyzed Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 |