Transgene Information

NamesyIs401 View on WormBase
Description[Phsp16.41::NLS::GAL4SK::VP64::let-858 3'UTR; Punc-122::RFP; 1kb DNA ladder(NEB)]

Strains carrying this transgene

Strain Genotype Species Description
PS7172 syIs337 syIs401 III. C. elegans syIs337 [15xUAS::Δpes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] III. syIs401 [hsp16.41p::NLS::GAL4SK::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder(NEB)].  syIs337 is a GFP cGAL effector. syIs401 is hsp-16.41 cGAL driver for heat shock promoter. Low levels of GFP fluorescence in a few head neurons without heat shock. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.