Transgene Information

NamesyIs396 View on WormBase
Description[pHW504(Punc-47::nls::GAL4SK::VP64::let-858 3'UTR), 25ng/L; Punc-122::RFP, 40ng/L; 1kb DNA ladder (NEB), 50ng/L]

Strains carrying this transgene

Strain Genotype Species Description
PS7167 syIs396 syIs337 III. C. elegans syIs396 [unc-47p::NLS::NLS::GAL4SK::VP64::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)].  syIs337 [15xUAS::Δpes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)].  syIs396 is unc-47 cGAL driver for GABAergic neurons.  syIs337 is GFP cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.