Transgene Information
| Name | dqSi1 View on WormBase |
|---|---|
| Description | [Pmex-5::ATX-2a::GFP::tbb-2 3' UTR + unc-119(+)] II was generated as follows: cDNA of the a isoform of atx-2 (atx-2a) was amplified from pT7 (generously provided by Rafal Ciosk, Friedrich Miescher Institute for Biomedical Research) and then inserted into pCR8/GW/TOPO (Invitrogen, Carlsbad, CA). The primers used to amplify the atx-2a were sense primer, 5'-ATGTCAACACCAACGGGTCTTCCTGCG-3', and antisense primer, 5'-CGGGGGGCTCTGAGAGTGCTG-3'. |
| Reporter | GFP |
Strains carrying this transgene
| Strain | Genotype | Species | Description |
|---|---|---|---|
| MAD63 | dqSi1 II; unc-119(ed3) III. | C. elegans | dqSi1 [mex-5p::atx-2a(cDNA)::GFP::tbb-2 3’UTR + unc-119(+)] II. Made by injection into strain EG6699; insertion confirmed by sequencing. dqSi1 rescues atx-2(ne4297). Reference: Gnazzo MM, et al. Mol Biol Cell. 2016 Oct 15;27(20):3052-3064. (PMID: 27559134) Del Castillo U, et al. Traffic. 2019 Jun;20(6):436-447. (PMID: 30989774) |