Transgene Information
| Name | adeIs1 View on WormBase |
|---|---|
| Description | |
| Reporter | GFP |
Strains carrying this transgene
| Strain | Genotype | Species | Description |
|---|---|---|---|
| ANA65 | adeIs1 II; unc-119(ed3) III. | C. elegans | adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. The transgene has been inserted on chromosome II, using the MosSCI technique. This strains expresses the SPD-1 protein fused to GFP in the germline (both males and females) and in embryos. SPD-1 is nucleolar in interphase and labels the central spindle in mitosis and meiosis, later accumulating at the midbody. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. doi: 10.1091/mbc.E14-12-1577. |
| ANA72 | adeIs1 II; unc-119(ed3) III; ltIs37 IV. | C. elegans | adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. SPD-1::GFP and mCherry-tagged histones allow visualisation of chromatin with central spindle/midbody during cell divisions. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. |