Transgene Information
| Name | wgIs457 View on WormBase |
|---|---|
| Description | WRM061C_F09(pRedFlp-Hgr)(nhr-149[21755]::S0001_pR6K_Amp_2xTY1ce_EGFP_FRT_rpsl_neo_FRT_3xFLAG)dFRT::unc-119-Nat |
| Reporter | EGFP |
Strains carrying this transgene
| Strain | Genotype | Species | Description |
|---|---|---|---|
| OP457 | unc-119(tm4063) III; wgIs457. | C. elegans | wgIs457 [nhr-149::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org) |