Transgene Information

NamewgIs432 View on WormBase
DescriptionWRM064A_D01(pRedFlp-Hgr)(zip-2[16701]::S0001_pR6K_Amp_2xTY1ce_EGFP_FRT_rpsl_neo_FRT_3xFLAG)dFRT::unc-119-Nat
ReporterEGFP

Strains carrying this transgene

Strain Genotype Species Description
OP432 unc-119(tm4063) III; wgIs432. C. elegans wgIs432 [zip-2::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)