Transgene Information

NameggIs1 View on WormBase
Description[nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
BB259 adr-1(uu49) I; adr-2(uu28) III; ggIs1 IV. C. elegans ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
BB278 adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; ggIs1 IV. C. elegans ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
YY178 ggIs1. C. elegans ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)]. Unknown if unc-119 is still in background. Reference: GuangS, et al. Nature. 2010 Jun 24;465(7301):1097-101.