Transgene Information

NamemaEx247 View on WormBase
Description[pat-3p::GFP::H2B::pat-3(mutated) 3'UTR + pat-3p::mCherry::H2B::pat-3 3'UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210)
ReportermCherry

Strains carrying this transgene

Strain Genotype Species Description
VT3145 unc-119(ed3) III; mir-83(n4638) IV; mir-34(gk437) X; maEx247. C. elegans maEx247 (pat-3p::GFP::H2B::pat-3(mutated) 3`UTR + pat-3p::mCherry::H2B::pat-3 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. DTC migration defects. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.
VT3178 unc-119(ed3) III; maEx247. C. elegans maEx247 (pat-3p::GFP::H2B::pat-3(mutated) 3`UTR + pat-3p::mCherry::H2B::pat-3 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.