Transgene Information

NamevkEx1244 View on WormBase
Description[nhx-2p::ubiquitin-Met::mCherry + myo-2p::GFP]
ReportermCherry

Strains carrying this transgene

Strain Genotype Species Description
VK1244 vkEx1244. C. elegans vkEx1244 [nhx-2p::ubiquitin-Met::mCherry + myo-2p::GFP]. mCherry behaves as an umodified cytosolic protein upon ubiquitin cleavage due to the absence of a degredation signal (N-terminal methionine). Diffuse mCherry expression in intestine. GFP+ pharynx. References: Miedel MT, et al. PLoS One. 2012;7(7):e40145. Gosai SJ, et al. PLoS One. 2010 Nov 12;5(11):e15460. Dantuma NP, et al. Nat Biotechnol. 2000 May;18(5):538-43.