Transgene Information

NameuIs46 View on WormBase
Description[rde-1p::mec-2(intron9)::rde-1) + ceh-22::GFP]

Strains carrying this transgene

Strain Genotype Species Description
TU3135 mec-8(u218) I; rde-1(ne219) V; uIs46. C. elegans uIs46 [rde-1p::mec-2(intron9)::rde-1) + ceh-22::GFP]. Temperature sensitive; maintain at 15 C. RNAi-sensitive at 15 C. Mechanosensory abnormal at 25 C. Uses the ninth intron of mec-2, whose splicing depends on mec-8, to produce temperature-sensitive expression and temperature-sensitive RNAi. Reference: Calixto et al. (2010) Nature Methods 7:407-11.