Transgene Information

NamemjIs144 View on WormBase
Description[mex-5p::HIS-58::GFP::piRNA(21UR-1)::tbb-2-3'UTR]

Strains carrying this transgene

Strain Genotype Species Description
SX1316 mjIs144 II; unc-119(ed3) III. C. elegans mjIs144 [mex-5p::GFP::his-58::21UR-1target::tbb-2 3'UTR + unc-119(+)] II. piRNA sensor strain. Single copy inserted into ttTi5605 (MosSCI). Superficially wild-type with loss of piRNA sensor silencing in piRNA pathway mutants (e.g. prg-1). GFP is silenced in wild-type, expressed in piRNA pathway mutants and can be used as a simple read-out for piRNA pathway function. Reference: Bagijn MP, et al. Science. 2012 Aug 3;337(6094):574-8.