Transgene Information

NameccEx7271 View on WormBase
Description[let-858::GFP + pha-1(+)]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
PD7271 pha-1(e2123) III; ccEx7271. C. elegans ccEx7271 [let-858::GFP + pha-1(+)]. This strain expresses nuclear-localized GFP in all somatic nuclei, but reduced or no GFP in germ cells. If maintained at 20C, pha-1(ts) genotype will select for transgenic animals. Sporadic germ cell expression can be observed when maintained at 25C.
SX340 unc-32(e189) mut-7(pk204) pha-1(e2123) III; ccEx7271. C. elegans ccEx7271 [let-858::GFP + pha-1(+)]. This strain expresses nuclear-localized GFP in all somatic nuclei, but reduced or no GFP in germ cells. If maintained at 20C, pha-1(ts) genotype will select for transgenic animals. Germ cell expression can be observed when maintained at 25C. Germline transgene silencing is abnormal.