Transgene Information

NamewgIs482 View on WormBase
DescriptionWRM062A_E02(pRedFlp-Hgr)(nhr-47[23960]::S0001_pR6K_Amp_2xTY1ce_EGFP_FRT_rpsl_neo_FRT_3xFLAG)dFRT::unc-119-Nat
ReporterEGFP

Strains carrying this transgene

Strain Genotype Species Description
OP482 unc-119(tm4063) III; wgIs482. C. elegans wgIs482 [nhr-47::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Maintain at 20 degrees. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)