Transgene Information

NamewgIs177 View on WormBase
Description[egl-27p::TY1::EGFP::3xFLAG + unc-119(+)]

Strains carrying this transgene

Strain Genotype Species Description
OP177 unc-119(ed3) III; wgIs177. C. elegans wgIs177 [egl-27::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of egl-27 coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (
SD1751 egl-27(we3) II; wgIs177. C. elegans wgIs177 [egl-27p::TY1::EGFP::3xFLAG + unc-119(+)]. Maintain at 20C or higher. Embryonic lethal at 15C. Reference: Xu X, Kim SK. PLoS Genet. 2012;8(12):e1003108.